Production, preliminary characterization and antitumor activity in vitro of polysaccharides from the mycelium of Pholiota dinghuensis Bi
Production, preliminary characterization and antitumor activity in vitro of polysaccharides from the mycelium of Pholiota dinghuensis Bi
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DOI：
10.1016/j.carbpol.2010.12.058
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发表时间：
2011-03-17
影响因子：
11.2
通讯作者：
Zeng, Xiaoxiong
中科院分区：
文献类型：
--
作者：
Gan, Dan;Ma, Liping;Zeng, Xiaoxiong
本文研究了定湖藻菌丝体多糖（PDP）的制备、初步表征及其体外抗肿瘤活性。首先，采用深层培养法制备了鼎湖菌菌丝体粗PDP。然后，通过DEAE-52纤维素和Sephadex G-100层析纯化粗PDP，得到三个纯化的PDP-1组分。PDP-2和PDP-3。我们发现PDP-3的单糖组成与PDP-1或PDP-2有很大的不同。此外，其蛋白质、硫酸盐和尿素酸的含量在所有多糖中最高。此外，PDP-3对人胃癌BGC-823细胞的体外抗增殖活性高于粗PDP。PDP-1或PDP-2。在浓度为400 mg/L、暴露时间为72 h时，对粗PDP、PDP-2和PDP-3的抑制率分别为69.36%、73.65%和85.78%。(C) 2010 Elsevier Ltd.版权所有。
The production, preliminary characterization and antitumor activity in vitro of mycelial polysaccharides from Pholiota dinghuensis Bi (PDP) were investigated in the present study. Firstly, crude PDP was prepared from the mycelia of P. dinghuensis Bi by submerged culture. Then, the crude PDP was purified by chromatography of DEAE-52 cellulose and Sephadex G-100, resulting three purified fractions of PDP-1. PDP-2 and PDP-3. We found that the monosaccharide composition of PDP-3 was greatly different from that of PDP-1 or PDP-2. In addition, it contained the highest contents of protein, sulfate and uronic acid among the polysaccharides tested. Furthermore, PDP-3 exhibited higher antiproliferative activity against human gastric cancer BGC-823 cells in vitro than crude PDP. PDP-1 or PDP-2. At a concentration of 400 mg/L and an exposure time of 72 h, the inhibition rates for crude PDP, PDP-2 and PDP-3 were 69.36%, 73.65% and 85.78%, respectively. (C) 2010 Elsevier Ltd. All rights reserved.