[PCR amplification of anaerobic fungal 18S rDNA from landfill sites].
[PCR amplification of anaerobic fungal 18S rDNA from landfill sites].
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发表时间：
2001-09
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通讯作者：
X. Hang;H. Yang;W. Corne
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作者：
X. Hang;H. Yang;W. Corne
基于核酸的技术被广泛用于表征环境样品中的微生物群落。在这项研究中，对7个垃圾渗滤液进行了18S rRNA基因的检测，这些基因来自合胞菌科的厌氧真菌。采用套式聚合酶链式反应(PCR)技术，从垃圾填埋场样品中扩增出18S rRNA基因片段。用第一对引物NS1/NS8和第二对引物Chyt-719/Chyt-1553对垃圾渗滤液进行扩增，所有的扩增产物经琼脂糖凝胶电泳法分析显示，有7个样品含有合胞菌科真菌。然后选择其中一个阳性结果进行PCR产物的克隆和测序，以确认扩增出的DNA的身份。本文提供的分子数据首次揭示了与厌氧瘤胃环境相关的合孢霉菌也存在于垃圾填埋场。
Nucleic acid based techniques are widely used to characterize microbial communities in environmental samples. In this study, seven landfill leachates were tested for the presence of 18S rRNA genes from anaerobic fungi from the family Chytridiomycetes. A nested Polymerase Chain Reaction (PCR) strategy was employed to amplify the 18S rRNA gene region of Chytridiomycetous fungi from landfill sites samples. All of the PCR products from landfill leachates amplified with the first primer pair NS1/NS8 and the second primer pair Chyt-719/Chyt-1553 were subjected to agarose gel electrophoresis to show seven samples containing fungi from the family Chytridiomycetes. One of the positive results was then selected for cloning and sequencing of the PCR product to confirm the identity of the amplified DNA. The molecular data presented here reveals for the first time that Chytridiomycetous fungi associated with the anaerobic rumen environment are also present in landfill sites.