Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation
Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in ginger and LC-MS/MS confirmation
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基于分子印迹聚合物的固相净化分析生姜中的赭曲霉毒素 A 并进行 LC-MS/MS 确认
DOI：
10.1016/j.foodcont.2013.03.023
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发表时间：
2013-10-01
期刊：
影响因子：
6
通讯作者：
Yang, Shihai
中科院分区：
文献类型：
--
作者：
Cao, Jiliang;Zhou, Shujun;Yang, Shihai
生姜是最常用的调味品和添加剂之一，也是广泛用于预防和治疗人类疾病的传统药物。然而，生姜容易受到赭曲霉毒素A（OTA）的污染，这是有毒，致癌和耐热的。建立了一种简单、可靠、低成本的分子印迹聚合物（MIP）固相萃取（SPE）超高效液相色谱-荧光检测（UPLC-FLR）测定生姜中OTA的方法。首先提取样品，然后用AFFINIMIP（R）SPE OTA柱净化，用于UPLC-FLR分析。在优化条件下，OTA的检测限和定量限分别为0.09和0.30 ng/mL。在生姜中添加5、15和25 μ g/kg的OTA，回收率为87.6%~ 94.5%。此外，在一个简单的再生程序后，基于MIP的SPE柱可以重复使用至少41次，获得超过80%的回收率的OTA生姜样品。将该方法应用于20批生姜样品的检测，其中6批样品中OTA含量低于最新法律的限量。(C)2013爱思唯尔有限公司保留所有权利。
Ginger, one of the most commonly used spices and additives, is also widely used to prevent and treat human disease as a traditional medicine. However, ginger is prone to contamination by ochratoxin A (OTA), which is toxic, carcinogenic and thermostable. Here, a simple, reliable and low-cost method based on molecularly imprinted polymer (MIP) as selective sorbent of solid-phase extraction (SPE) was proposed for the determination of OTA in ginger by ultra-performance liquid chromatography coupled with fluorescence detection (UPLC-FLR). The samples were firstly extracted and then cleaned up with an AFFINIMIP (R) SPE OTA column for UPLC-FLR analysis. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) for OTA were 0.09 and 0.30 ng mL(-1), respectively. The recoveries of OTA from ginger spiked at 5,15 and 25 mu g kg(-1) ranged from 87.6 to 94.5%. In addition, after a simple regenerated procedure, the MIP-based SPE column could be reused at least forty-one times to obtain more than 80% recoveries of OTA for ginger samples. The developed method was applied to the detection of twenty batches of ginger and six samples were contaminated by OTA with levels below the newest legal limits. (C) 2013 Elsevier Ltd. All rights reserved.