A reusable neurotransmitter aptasensor for the sensitive detection of serotonin
A reusable neurotransmitter aptasensor for the sensitive detection of serotonin
复制标题
一种可重复使用的神经递质适体传感器，用于灵敏检测血清素
DOI：
10.1016/j.aca.2020.11.010
复制
发表时间：
2021-01-13
影响因子：
6.2
通讯作者：
Du, Jiangyan
中科院分区：
文献类型：
--
作者：
Geng, Xue;Zhang, Mengtian;Du, Jiangyan
血清素是人体神经系统重要的神经递质之一，与中枢神经系统疾病有关。在此，我们利用预先设计和制备的DNA适体制备了一种新型电化学适体传感器，用于快速、灵敏地检测血清素。在没有血清素的情况下，由于适体的发夹结构疏松，MB可以更接近电极表面，因此适体传感器上的电子转移速率比有血清素的情况更快。当血清素存在时，适体的发夹结构被延长，MB远离电极表面。通过比较适体中间MB标记的适体传感器与适体3'端MB标记的适体传感器的灵敏度，讨论了MB标记位点对所提出的适体传感器分析性能的影响。研究发现，由于适体与血清素结合前后发生了特定的构象变化，中间标记的适体传感器的灵敏度远高于末端标记的适体传感器。开发的适体传感器在测定血清素方面表现出快速的电化学响应和高灵敏度。在最佳实验条件下，中间标记适体传感器检测血清素浓度的线性范围为1 pM-10 nM，检测限为0.017 fM（S/N = 3）。此外，所提出的适体传感器可重复使用，对100倍稀释的大鼠脑脊液中血清素的检测显示出良好的重现性和选择性，在实际样品中血清素的检测中具有良好的应用前景。 (C) 2020 Elsevier B.V. 保留所有权利。
Serotonin is one of the important neurotransmitters in human nervous system and associated with central nervous system diseases. Herein, we have prepared a novel electrochemical aptasensor for rapid and sensitive detection of serotonin by using the pre-designed and prepared DNA aptamers. In the absence of serotonin, the electron transfer rate on the aptasensor was faster than that in the presence of serotonin due to the hairpin structure of the aptamer was loose and MB could be closer to the electrode surface. While in the presence of serotonin, the hairpin structure of the aptamer was extended and MB was far away from the electrode surface. The effect of MB labeled sites on analytical performances of the proposed aptasensors was discussed by comparing sensitivity of the aptasensors that MB labeled in the intermediate of the aptamer with that MB labeled at the 3' end of the aptamer. It was found that sensitivity of the intermediate-labeled aptasensor was much higher than the terminal-labeled aptasensor due to the specific conformational changes before and after aptamer binding to serotonin. The developed aptasensors exhibits a rapid electrochemical response and high sensitivity for the determination of serotonin. Under the optimal experimental conditions, the linear range for serotonin concentrations by the intermediate-labeled aptasensor was 1 pM-10 nM with a detection limit of 0.017 fM (S/ N = 3). Moreover, the proposed aptasensor is reusable and shows good reproducibility and selectivity for the detection of serotonin in 100-fold diluted rat cerebrospinal fluid, suggesting a good application prospect in the detection of serotonin in real samples. (C) 2020 Elsevier B.V. All rights reserved.