Mechanically strong double network photocrosslinked hydrogels from N,N-dimethylacrylamide and glycidyl methacrylated hyaluronan
Mechanically strong double network photocrosslinked hydrogels from N,N-dimethylacrylamide and glycidyl methacrylated hyaluronan
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DOI：
10.1016/j.biomaterials.2008.01.012
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发表时间：
2008-05-01
期刊：
影响因子：
14
通讯作者：
Chen, Weiliam
中科院分区：
文献类型：
--
作者：
Weng, Lihui;Gouldstone, Andrew;Chen, Weiliam
透明质酸(HA)是一种广泛存在于生物组织中的天然多糖，可对其进行修饰以制备生物材料。本工作首先将甲基丙烯酸缩水甘油酯修饰的羟基磷灰石(HA)光交联形成第一网络(PHA)，然后将第二网络光交联的DAAM引入PHA网络，得到一系列具有高机械强度的高孔PHA/N，N-二甲基丙烯酰胺(DAAM)水凝胶(PHA/DAAM)。由于双网络结构的协同作用，尽管PHA/DAAM水凝胶的含水率为90%，但其压缩弹性系数和断裂应力分别大于0.5和5.2 Mpa。与光交联型透明质酸单网络水凝胶相比，PHA/DAAM水凝胶一般非常脆，容易断裂，但PHA/DAAM水凝胶具有延展性。以小鼠真皮成纤维细胞为模型细胞，验证了PHA/DAAM水凝胶的体外无细胞毒性。细胞在这些水凝胶表面沉积细胞外基质，天狼星红对I型胶原的阳性染色证实了这一点。PHA/DAAM水凝胶还具有抗生物降解性，即使在与成纤维细胞共培养2个月后，也基本保持了其优异的力学性能。(C)2008爱思唯尔有限公司。保留所有权利。
Hyaluronan (HA) is a natural polysaccharide abundant in biological tissues and it can be modified to prepare biomaterials. In this work, HA modified with glycidyl methacrylate was photocrosslinked to form the first network (PHA), and then a series of highly porous PHA/N, N-dimethylacrylamide (DAAm) hydrogels (PHA/DAAm) with high mechanical strength were obtained by incorporating a second network of photocrosslinked DAAm into PHA network. Due to the synergistic effect produced by double network (DN) structure, despite containing, 90% of water, the resulting PHA/DAAm hydrogel showed a compressive modulus and a fracture stress over 0.5 MPa and 5.2 MPa, respectively. Compared to the photocrosslinked hyaluronan single network hydrogel, which is generally very brittle and fractures easily, the PHA/DAAm hydrogels are ductile. Mouse dermal fibroblast was used as a model cell line to validate in vitro non-cytotoxicity of the PHA/DAAm hydrogels. Cells deposited extracellular matrix on the surface of these hydrogels and this was confirmed by positive staining of Type I collagen by Sirius Red. The PHA/DAAm hydrogels were also resistant to biodegradation and largely retained their excellent mechanical properties even after 2 months of co-culturing with fibroblasts. (C) 2008 Elsevier Ltd. All rights reserved.