Towards the modulation of oxidative damage, apoptosis and tight junction protein in response to dietary leucine deficiency: A likely cause of ROS-induced gill structural integrity impairment
Towards the modulation of oxidative damage, apoptosis and tight junction protein in response to dietary leucine deficiency: A likely cause of ROS-induced gill structural integrity impairment
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调节氧化损伤、细胞凋亡和紧密连接蛋白以应对饮食亮氨酸缺乏：ROS诱导的鳃结构完整性损伤的可能原因
DOI：
10.1016/j.fsi.2017.09.049
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发表时间：
2017-11-01
影响因子：
4.7
通讯作者：
Feng, Lin
中科院分区：
文献类型：
--
作者：
Jiang, Wei-Dan;Deng, Yu-Ping;Feng, Lin
本研究探讨了亮氨酸对草鱼鳃抗氧化状态、细胞凋亡和紧密连接损伤的保护作用。试验用6个亮氨酸水平（7.1、8.9、11.0、133、15.2和17.1g/kg）的饲料饲喂草鱼8周。每天投喂4次鱼至明显饱食。结果表明，与亮氨酸缺乏组相比，8.9- 11.3g亮氨酸/kg饲料组能降低蛋白质羰基（PC）、丙二醛（MDA）和活性氧（ROS）含量，这可能与其提高羟自由基和抗超氧阴离子自由基能力，改善鳃的抗氧化状态有关。谷胱甘肽含量以及Cu/Zn超氧化物歧化酶（SOD 1）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、谷胱甘肽-S-转移酶（GST）和谷胱甘肽还原酶（GR）的活性和mRNA水平，即核因子红细胞2相关因子2（Nrf 2）mRNA表达上调。此外，亮氨酸缺乏通过上调caspase-3、caspase-8和caspase-9的表达，下调雷帕霉素靶蛋白和核糖体S6蛋白激酶1的表达，诱导DNA片段化。此外，亮氨酸缺乏增加白细胞介素-1 β（IL-1 β）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）mRNA表达，降低IL-10和转化生长因子β（TGF-β），这部分与核因子κ B（NF-κ B）及其抑制剂（I κ B）相关。与此相反，IL-1，IL-8和TNF-α的相对mRNA表达下调与8.9-113 g亮氨酸kg（-1）的饮食表达。最后，在亮氨酸饮食条件下，紧密连接蛋白claudin、紧密连接小带1、claudin B、claudin 3和claudin 12的相对mRNA表达量上调。我们的研究结果表明，亮氨酸保护鱼鳃的结构完整性部分是因为抑制细胞凋亡，抗氧化状态的改善，在鱼鳃的紧密连接蛋白和相关信号分子的mRNA表达的调节。(C)2017爱思唯尔有限公司版权所有
The current study explored the protective effect of leucine on antioxidant status, apoptosis and tight junction damage in the gill of grass carp (Ctenopharyngodon idella Val.). The trial was conducted by feeding grass carp with six graded level of leucine (7.1, 8.9, 11.0, 133, 15.2 and 17.1 g kg(-1) diet) for 8 weeks. The fish were fed to apparent satiation 4 times per day. The results indicated that compared with the leucine deficiency group, 8.9-11.3 g leucine kg(-1) diet supplementations decreased protein carbonyl (PC), malondialdehyde (MDA) and ROS contents, which may be partially attributed to the improvement of antioxidant status in the gill by increasing hydroxyl radical capacity and anti-superoxide radicals, glutathione contents and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), glutathione peroxidase (GPx), catalase (CAT), glutathione-S-transferase (GST) and glutathione reductase (GR), that referring to the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression. Moreover, leucine deficiency induced DNA fragmentation via the up-regulation of caspase-3, caspase-8 and caspase-9 expressions and down-regulation of target of rapamycin and ribosomal S6 protein kinase 1 expressions. Furthermore, leucine deficiency increased interleukin-1 beta (IL-1 beta), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) mRNA expression and decreased IL-10 and transforming growth factor beta (TGF-beta), which was partly related to nuclear factor kappa B (NF-kappa B) and its inhibitor (I kappa B). In contrast, the relative mRNA expression of IL-1, IL-8 and TNF-alpha was down-regulated with 8.9-113 g leucine kg(-1) diet supplementations. Finally, the relative mRNA expression of tight junction protein, including occludin, zonula occludens-1, claudin b, claudin 3 and claudin 12 was up-regulated with leucine diet supplementations. Our results indicate that leucine protected the fish gill structural integrity partially because of the inhibition of apoptosis, the improvement of antioxidant status, the regulation of tight junction protein and related signalling molecules mRNA expressions in the fish gill. (C) 2017 Elsevier Ltd. All rights reserved.