BEHAVIORAL CIRCADIAN-RHYTHMS MEASURED IN REAL-TIME BY AUTOMATIC IMAGE-ANALYSIS - APPLICATIONS IN PARASITOID INSECTS
BEHAVIORAL CIRCADIAN-RHYTHMS MEASURED IN REAL-TIME BY AUTOMATIC IMAGE-ANALYSIS - APPLICATIONS IN PARASITOID INSECTS
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DOI：
10.1111/j.1365-3032.1994.tb01067.x
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发表时间：
1994-03-01
影响因子：
1.5
通讯作者：
BOULETREAU, M
中科院分区：
文献类型：
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作者：
ALLEMAND, R;POMPANON, F;BOULETREAU, M
开发了视频分析系统来研究昆虫的行为昼夜节律。在图像分析和机器人技术的使用方面进行了技术创新。在图像分析中，昆虫的自动检测和多个变量（线速度和角速度、运动活动等）的计算都是实时进行的，这节省了时间并允许长期研究而无需操作员的任何干预。通过机器人技术移动相机可以同时研究许多个体（即 60 个个体用于研究成年昆虫的运动活动，400 多个个体用于研究成年寄生蜂从宿主中的出现）。介绍了这种新设备的四种应用。他们研究寄生昆虫的昼夜节律：运动活动和运动参数的时间变化，成虫运动活动出现和开始的每日分布。讨论了该系统对几种实验材料和情况的适应性，并强调了在宿主-寄生物关联方法中研究行为节律的兴趣。
A video analysis system was developed to study behavioural circadian rhythms in insects. Technical innovations were performed in image analysis and in the use of robotics. In image analysis, both the automatic detection of the insect and the calculation of several variables (linear and angular speed, locomotor activity, etc.) are carried out in real-time, which saves time and allows long-term studies without any intervention of the operator. The displacement of the camera by robotics allows the simultaneous study of many individuals (i.e. sixty individuals for the study of locomotor activity of adult insects, more than 400 individuals for the study of the emergence of adult parasitoids from their host). Four applications of this new device are presented. They deal with circadian rhythms in parasitoid insects: locomotor activity and time variation in locomotory parameters, daily distribution of the emergence and onset of the locomotor activity in adults. The adaptability of the system to several experimental materials and situations is discussed and the interest of the study of behavioural rhythms in the approach of host-parasitoid associations is emphasized.