The role of flagella, but not fimbriae, in the adherence of Salmonella enterica serotype Enteritidis to chick gut explant
The role of flagella, but not fimbriae, in the adherence of Salmonella enterica serotype Enteritidis to chick gut explant
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DOI：
10.1099/00222615-48-8-771
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发表时间：
1999-08-01
影响因子：
3
通讯作者：
Woodward, MJ
中科院分区：
文献类型：
--
作者：
Allen-Vercoe, E;Woodward, MJ
为了了解菌毛和鞭毛在肠炎沙门氏菌在鸡体内黏附和定植中的作用，建立了一种体外肠道黏附试验，并用于评估一株肠炎沙门氏菌野生型菌株和同基因非菌毛和非鞭毛突变菌株的黏附。鸡传染性肠炎临床分离株S1400/94具有编码1型、SEF14、SEF17、质粒型和长极菌毛的基因。无法阐明这些菌毛的突变菌株是通过等位基因交换产生的。每个菌毛操纵子通过插入抗生素抗性基因盒而失活。此外，分别编码鞭毛主要亚单位、运动能量转换系统和趋化信号蛋白之一的flc、motAB和chea基因座也被类似地失活。非鞭毛突变菌株的粘附性明显低于野生型菌株，而突变菌株与野生型菌株一样不能形成所附着的任何类型的菌毛。有鞭毛但不活动(麻痹)的突变株和游泳性趋化缺陷突变株的粘附性低于野生型菌株，但观察结果取决于所用的检测条件。
To gain an understanding of the role of fimbriae and flagella in the adherence and colonisation of Salmonella enterica serotype Enteritidis in chickens, an in-vitro gut adherence assay was developed and used to assess the adherence of a wild-type Enteritidis strain and isogenic non-fimbriate and non-flagellate mutant strains. Enteritidis strain S1400/94, a clinical isolate virulent in chickens, was shown to possess genes which encoded type 1, SEF14, SEF17, plasmid-encoded and long polar fimbriae. Mutant strains unable to elaborate these fimbriae were created by allelic exchange. Each fimbrial operon was inactivated by the insertion of an antibiotic resistance gene cassette. In addition, fliC, motAB and cheA loci, which encode the major subunit of the flagellum, the energy-translation system for motility and one of the chemotaxis signalling proteins, respectively, were similarly inactivated. Non-flagellate mutant strains were significantly less adherent than the wild-type strain, whereas mutant strains defective for the elaboration of any of the types of fimbriae adhered as well as the wild-type strain. A flagellate but non-motile (paralysed) mutant strain and a smooth-swimming chemotaxis-deficient mutant strain were shown to be less adherent than the wild-type strain, but that observation depended on the assay conditions used.