Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes
Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes
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DOI：
10.1182/blood.v90.3.952.952_952_957
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发表时间：
1997-08-01
期刊：
影响因子：
20.3
通讯作者：
Sadelain, M
中科院分区：
文献类型：
--
作者：
Gallardo, HF;Tan, C;Sadelain, M
有效地产生大量可靠表达外源基因的人原代T细胞对于研究T细胞功能和改善过继细胞疗法至关重要。这一目标是通过使用重组逆转录病毒假型与任何一个巨猿白血病病毒（GaLV）包膜或水泡性口炎病毒G（VSV-G）糖蛋白。我们在这里表明，这两种逆转录病毒颗粒介导稳定的基因转移在CD 4（+）和CD 8（+）外周血淋巴细胞培养在优化条件下。然而，VSV-G假型病毒体可能会导致转导假象，必须小心排除。VSV-G病毒体需要10至100倍高浓度的感染性颗粒，以实现与GaLV病毒体相当的基因转移水平。尽管如此，VSV-G包被颗粒的物理稳定性使得病毒原液的浓度能够达到每毫升10（9）个感染性颗粒或更多。(C)1997年，美国血液学会。
It is essential for the study of T-cell function and the improvement of adoptive cell therapies to efficiently generate large populations of human primary T cells that reliably express foreign genes. This goal is achieved by using recombinant retroviruses pseudotyped with either the gibbon ape leukemia virus (GaLV) envelope or the vesicular stomatitis virus G (VSV-G) glycoprotein. We show here that both retroviral particles mediate stable gene transfer in CD4(+) and in CD8(+) peripheral blood lymphocytes cultured under optimized conditions. However, VSV-G-pseudotyped virions may cause transduction artifacts that must be carefully excluded. The VSV-G virions require 10- to 100-fold higher concentrations of infectious particles to achieve levels of gene transfer comparable to GaLV-virions. Nonetheless, the physical stability of VSV-G-coated particles enables the concentration of viral stocks to 10(9) infectious particles per milliliter or more. (C) 1997 by The American Society of Hematology.