Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands
Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands
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DOI：
10.1128/jcm.01288-15
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发表时间：
2016-02-01
影响因子：
9.4
通讯作者：
Wagenaar, Jaap A.
中科院分区：
文献类型：
--
作者：
Duim, Birgitta;Verstappen, Koen M.;Wagenaar, Jaap A.
耐甲氧西林假中间葡萄球菌 (MRSP) 通常具有多重耐药性 (MDR)，最近已成为全球犬类健康的威胁。了解特定 MRSP 谱系的时间分布、其抗菌素耐药表型及其与临床状况的关联可能有助于我们了解 MRSP 在狗中的出现和传播。本研究的目的是确定荷兰每年 MRSP 谱系的比例及其抗菌素耐药表型，并检查与临床状况的可能关联。 MRSP 首次从 2004 年提交给乌得勒支大学兽医学院进行诊断的犬样本中分离出来。伪中间链球菌中 MRSP 的年累积发病率从 2004 年的 0.9% 增加到 2013 年的 7%。MRSP 与脓皮病显着相关，在较小程度上与伤口感染和外耳炎相关。 478 个 MRSP 分离株的多位点序列分型 (MLST) 产生了属于 4 个克隆复合体 (CC) 的 39 个序列类型 (ST) 和 15 个单株。 CC71 是自 2004 年以来出现的主要谱系，CC258、CC45 和几个不相关的分离株在接下来的几年中变得更加常见。除两个菌株外，所有菌株均具有 MDR 表型，但属于 CC258 或单株的菌株耐药性较低。总之，我们的研究表明，从 2004 年起，MDR CC71 成为主要谱系，而耐药性较低的谱系正在部分取代 CC71。
Methicillin-resistant Staphylococcus pseudintermedius (MRSP), which is often multidrug resistant (MDR), has recently emerged as a threat to canine health worldwide. Knowledge of the temporal distribution of specific MRSP lineages, their antimicrobial resistance phenotypes, and their association with clinical conditions may help us to understand the emergence and spread of MRSP in dogs. The aim of this study was to determine the yearly proportions of MRSP lineages and their antimicrobial-resistant phenotypes in the Netherlands and to examine possible associations with clinical conditions. MRSP was first isolated from a canine specimen submitted for diagnostics to the Faculty of Veterinary Medicine of Utrecht University in 2004. The annual cumulative incidence of MRSP among S. pseudintermedius increased from 0.9% in 2004 to 7% in 2013. MRSP was significantly associated with pyoderma and, to a lesser extent, with wound infections and otitis externa. Multilocus sequence typing (MLST) of 478 MRSP isolates yielded 39 sequence types (ST) belonging to 4 clonal complexes (CC) and 15 singletons. CC71 was the dominant lineage that emerged since 2004, and CC258, CC45, and several unlinked isolates became more frequent during the following years. All but two strains conferred an MDR phenotype, but strains belonging to CC258 or singletons were less resistant. In conclusion, our study showed that MDR CC71 emerged as the dominant lineage from 2004 and onward and that less-resistant lineages were partly replacing CC71.