B-lymphocyte responses to trinitrophenyl-conjugated Ficoll: requirement for T lymphocytes and Ia-bearing adherent cells.
B-lymphocyte responses to trinitrophenyl-conjugated Ficoll: requirement for T lymphocytes and Ia-bearing adherent cells.
复制标题
B 淋巴细胞对三硝基苯基共轭聚蔗糖的反应：对 T 淋巴细胞和 Ia 贴壁细胞的要求。
DOI：
10.1073/pnas.78.8.5113
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发表时间：
1981
影响因子：
11.1
通讯作者：
Germain,RN
中科院分区：
文献类型：
--
作者：
Letvin,NL;Benacerraf,B;Germain,RN
进行这些研究以表征B淋巴细胞对半抗原化多糖三硝基苯基缀合的Ficoll应答的细胞需求。通过使用体外微量培养系统，证明了B淋巴细胞的半抗原特异性抗三硝基苯基缀合的Ficoll空斑形成细胞应答需要带有Ia的粘附辅助细胞和Thy 1+ Lyt 1+2-尼龙羊毛非粘附（T）淋巴细胞。这种T细胞可以在体内用非衍生化的Ficoll引发，以显示载体特异性辅助细胞功能，用于体外衍生化的Ficoll应答。这些研究结果的影响，我们的理解B淋巴细胞触发所谓的T-非依赖性抗原进行了讨论。
These studies were done to characterize the cellular requirements for B-lymphocyte responses to the haptenated polysaccharide trinitrophenyl-conjugated Ficoll. By using an in vitro microculture system, it was demonstrated that hapten-specific anti-trinitrophenyl-conjugated Ficoll plaque-forming cell responses by B lymphocytes require Ia-bearing adherent accessory cells and Thy 1+ Lyt 1+2- nylon wool-nonadherent (T) lymphocytes. Such T cells could be primed in vivo with nonderivatized Ficoll to show carrier-specific helper cell function for derivatized Ficoll responses in vitro. The implications of these findings for our understanding of b-lymphocyte triggering by so-called T-independent antigens are discussed.