Nontargeted Screening and Determination of Sulfonamides: A Dispersive Micro Solid-Phase Extraction Approach to the Analysis of Milk and Honey Samples Using Liquid Chromatography-High-Resolution Mass Spectrometry
Nontargeted Screening and Determination of Sulfonamides: A Dispersive Micro Solid-Phase Extraction Approach to the Analysis of Milk and Honey Samples Using Liquid Chromatography-High-Resolution Mass Spectrometry
复制标题
磺胺类药物的非靶向筛选和测定：使用液相色谱-高分辨率质谱分析牛奶和蜂蜜样品的分散微量固相萃取方法
DOI：
10.1021/acs.jafc.6b05773
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发表时间：
2017-03-08
影响因子：
6.1
通讯作者：
Yan, Pengcheng
中科院分区：
文献类型：
--
作者：
Hu, Shuping;Zhao, Min;Yan, Pengcheng
建立了一种基于分散微量固相萃取（分散微量固相萃取）结合液相色谱高分辨质谱（LC-HRMS）的简单、快速、灵敏、选择性和环境友好的方法来分析蜂蜜和牛奶中的磺胺类药物。设计了一种有效的非靶向筛选策略，使用全 MS/全离子碎片 (AlF) 质谱采集模式来发现和鉴定蜂蜜和牛奶中已知和未知的磺酰胺。对分散微量SPE对萃取效率的实验参数和条件进行了详细优化。在优化条件下，分散微量SPE方法对牛奶中目标磺胺类药物的检出限为0.003~0.2μg/L，蜂蜜中目标磺胺类药物的检出限为0.01~1μg/kg，回收率在68.8%~115.8%之间。与报道的方法相比，本研究在方便性、低成本和环境友好方面取得了进步。
A simple, rapid, sensitive, selective, and environmentally friendly method, based on a dispersive micro solid-phase extraction approach (dispersive micro SPE) coupled with liquid chromatography high-resolution mass spectrometry (LC-HRMS), was established for the analysis of sulfonamides in honey and milk. An efficient nontargeted screening strategy was designed to discover and identify known and unknown sulfonamides in honey and milk using full-MS/all ion fragmentation (AlF) mass spectrometry acquisition mode. The experimental parameters and conditions of dispersive micro SPE on extraction efficiency were optimized in detail. Under the optimized conditions, the dispersive micro SPE method showed a low limit of detection (LOD) for the targeted sulfonamides ranging from 0.003 to 0.2 mu g/L in milk and from 0.01 to 1 mu g/kg in honey with the recoveries of the analytes between 68.8 and 115.8%. Compared with the reported methods, improvements in convenience, low cost, and environmental friendliness were obtained in this study.