Oscillatory Reaction Induced Periodic C-Quadruplex DNA Gating of Artificial Ion Channels.
Oscillatory Reaction Induced Periodic C-Quadruplex DNA Gating of Artificial Ion Channels.
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DOI：
10.1021/acsnano.6b08727
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发表时间：
2017-02
期刊：
影响因子：
17.1
通讯作者：
Jian Wang;Ruochen Fang;Jue Hou;Huacheng Zhang;Ye Tian;Huanting Wang;Lei Jiang
中科院分区：
文献类型：
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作者：
Jian Wang;Ruochen Fang;Jue Hou;Huacheng Zhang;Ye Tian;Huanting Wang;Lei Jiang
生物体内许多受生化反应控制的离子通道具有自主的、周期性的门控功能，在生命系统的连续物质传输和信号传递中起着重要作用。受这些功能性生物离子通道系统的启发，在这里，我们报告了一种人工自振荡纳米通道系统，该系统可以在恒定条件下自主地和周期性地控制其门控过程。该系统是通过将由BrO 3-、Fe（CN）64-、H+和SO 32-组成的化学振荡器整合到用C4 DNA马达修饰的合成质子敏感纳米通道中来构建的。化学振荡器，包含H+-产生和H+-消耗反应，可以周期性地驱动通道壁上的C4-DNA马达在无规卷曲和折叠的i-基序结构之间的构象变化，从而导致纳米通道在开放和闭合状态之间的自主门控。通过在恒定反应条件下监测的通道的周期性高低离子电流振荡证实了自主门控过程。利用与DNA分子集成的化学振荡器代表了一种将振荡反应的化学能直接转换为人工纳米通道构象变化的动能的方法，甚至可以在人工纳米流体装置中实现多种自主门控功能。
Many biological ion channels controlled by biochemical reactions have autonomous and periodic gating functions, which play important roles in continuous mass transport and signal transmission in living systems. Inspired by these functional biological ion channel systems, here we report an artificial self-oscillating nanochannel system that can autonomously and periodically control its gating process under constant conditions. The system is constructed by integrating a chemical oscillator, consisting of BrO3-, Fe(CN)64-, H+, and SO32-, into a synthetic proton-sensitive nanochannel modified with C-quadruplex (C4) DNA motors. The chemical oscillator, containing H+-producing and H+-consuming reactions, can cyclically drive conformational changes of the C4-DNA motors on the channel wall between random coil and folded i-motif structures, thus leading to autonomous gating of the nanochannel between open and closed states. The autonomous gating processes are confirmed by periodic high-low ionic current oscillations of the channel monitored under constant reaction conditions. The utilization of a chemical oscillator integrated with DNA molecules represents a method to directly convert chemical energy of oscillating reactions to kinetic energy of conformational changes of the artificial nanochannels and even to achieve diverse autonomous gating functions in artificial nanofluidic devices.