SEQUENCE SIMILARITY BETWEEN THE MAMMALIAN BMI-1 PROTOONCOGENE AND THE DROSOPHILA REGULATORY GENES PSC AND SU(Z)2
SEQUENCE SIMILARITY BETWEEN THE MAMMALIAN BMI-1 PROTOONCOGENE AND THE DROSOPHILA REGULATORY GENES PSC AND SU(Z)2
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DOI：
10.1038/353353a0
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发表时间：
1991-09-26
期刊：
影响因子：
64.8
通讯作者：
BERNS, A
中科院分区：
文献类型：
--
作者：
VANLOHUIZEN, M;FRASCH, M;BERNS, A
Bmi-1原癌基因可被Moloney小鼠白血病前病毒插入E-MU-myc转基因小鼠1，2激活。它编码一个高度保守的核蛋白，由324个氨基酸组成，属于一个可能含有新的锌指的蛋白家族。该家族的另一个密切相关的成员是小鼠蛋白MEL-18(参考文献)。3)。在这里，我们报道了一个来自果蝇的同源基因(D-BMI)的克隆和鉴定。我们的分析表明，小鼠BMI-1蛋白的不同结构域，包括假定的锌指基序，在更大的D-BMI蛋白中高度保守。染色体定位和序列比较表明，D-BMI与后天性梳(PSC)4-6相同，表明小鼠BMI和PSC之间的保守结构域也在Zust(SU(Z)2)6-8的抑制子-2中保守。
THE bmi-1 proto-oncogene can be activated by Moloney murine leukaemia proviral insertions in E-mu-myc transgenic mice 1,2. It encodes a highly conserved nuclear protein of 324 amino acids which belongs to a family of proteins containing a putative new zinc-finger. Another closely related member of this family is the mouse protein Mel-18 (ref. 3). Here we report on the cloning and characterization of a homologous gene (D-bmi) from Drosophila melanogaster. Our analysis indicates that distinct domains of the mouse Bmi-1 protein, including the putative zinc-finger motif, are highly conserved within the much larger D-Bmi protein. Chromosomal localization and sequence comparison reveal that D-bmi is identical to Posterior Sex Combs (Psc) 4-6 and indicate that the conserved domains between mouse bmi and Psc are also conserved within Suppressor-2 of Zeste (Su(z)2) 6-8.