Exposure to lambda-cyhalothrin, a synthetic pyrethroid, increases reactive oxygen species production and induces genotoxicity in rat peripheral blood
Exposure to lambda-cyhalothrin, a synthetic pyrethroid, increases reactive oxygen species production and induces genotoxicity in rat peripheral blood
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DOI：
10.1177/0748233713475516
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发表时间：
2015-05-01
影响因子：
1.9
通讯作者：
Gdoura, Radhouane
中科院分区：
文献类型：
--
作者：
Fetoui, Hamadi;Feki, Ameni;Gdoura, Radhouane
高效氯氟氰菊酯（LTC）是一种合成拟除虫菊酯，具有广谱杀虫和杀螨活性，用于控制各种昆虫害虫。然而，人们对它的不良影响知之甚少，特别是与人类遗传毒性有关的影响。为了阐明LTC的遗传毒性机制，对微核（MN）频率、活性氧（ROS）水平、红细胞渗透脆性、亚硝酸盐（NO）形成、蛋白质羰基（PCO）水平和丙二醛（MDA）产生进行了为期7、14和21天的评价。我们的研究结果表明，大鼠暴露于LTC（1/10 DL 50 = 6.23 mg/kg）的时间为7，14和21天诱导大鼠外周血中明显的遗传毒性效应，仅在治疗的第21天，MN的频率显着增加。两组红细胞渗透脆性差异有统计学意义。此外，在处理的不同时间观察到红细胞中ROS含量、NO形成、PCO水平和脂质过氧化反应的显著增加（p < 0.01），表明氧化应激在其毒性中的含义。这些结果证实了LTC在大鼠外周血中的遗传毒性和促氧化作用。
Lambda-cyhalothrin (LTC) is a synthetic pyrethroid with a broad spectrum of insecticidal and acaricidal activities used to control a wide range of insect pests in a variety of applications. However, there is little known about its adverse effects, in particular those related to its genotoxicity in humans. To elucidate the genotoxicity mechanisms of LTC, the micronuclei (MN) frequencies, the levels of reactive oxygen species (ROS), erythrocyte osmotic fragility, nitrite (NO) formation, protein carbonyl (PCO) levels and malondialdehyde (MDA) production were evaluated for a period of 7, 14 and 21 days. Our results show that exposure rat to LTC (1/10DL50 = 6.23 mg/kg) for a period of 7, 14 and 21 days induced a noticeable genotoxic effect in rat peripheral blood evidenced by a significant increase in the frequency of MN only at day 21 of treatment. Significant differences between the two groups were observed in erythrocyte osmotic fragility. Further, a significant (p < 0.01) increase in ROS contents, NO formation, PCO levels and lipid peroxidation in erythrocytes were observed at different times of treatments, suggesting the implication of oxidative stress in its toxicity. These results confirm the genotoxic and the pro-oxidant effects of LTC in rat peripheral blood.