REGULATION OF SERPIN GENE-1 IN MANDUCA-SEXTA
REGULATION OF SERPIN GENE-1 IN MANDUCA-SEXTA
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DOI：
10.1016/0965-1748(94)00067-r
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发表时间：
1995-02-01
影响因子：
3.8
通讯作者：
WILLOTT, E
中科院分区：
文献类型：
--
作者：
KANOST, MR;PRASAD, SV;WILLOTT, E
六钩鱼血淋巴中含有丝氨酸蛋白酶抑制剂--丝氨酸蛋白酶超家族的蛋白质。我们已经使用了针对msexta serpin基因-1mRNA和蛋白的探针来研究该基因的表达和激素调节。Serpin基因-1在幼虫脂肪体中高水平表达，在血细胞中低丰度表达，而Serpin蛋白定位于颗粒细胞颗粒中。摄食的4龄和5龄幼虫的脂肪体中含有丰富的Serpin基因-1mRNA，但在蜕皮和游走期突然消失。发育过程中血淋巴中SERPIN蛋白的浓度与脂肪体中SERPIN mRNA的丰度有关。体内和体外实验结果表明，20-羟基蜕皮酮对丝氨酸基因-1具有负调控作用。
Hemolymph of Manduca sexta contains proteins from the serpin superfamily, which are inhibitors of serine proteinases. We have used probes specific for M. sexta serpin gene-1 mRNA and protein to study the expression and hormonal regulation of this gene. Serpin gene-1 is expressed at a high level in larval fat body and at a lower abundance in hemocytes, where serpin protein is localized in the granules of granular cells. Serpin gene-1 mRNA is abundant in the fat body of feeding fourth and fifth instar larvae, but disappears abruptly at molts and at the wandering stage. The concentration of serpin proteins in hemolymph during development is correlated with the abundance of serpin mRNA in fat body. Results of in vivo and in vitro experiments indicate that 20-hydroxyecdysone has a role in negative regulation of serpin gene-1.