Bacterial communities in manures of piglets and adult pigs bred with different feeds revealed by 16S rDNA 454 pyrosequencing
Bacterial communities in manures of piglets and adult pigs bred with different feeds revealed by 16S rDNA 454 pyrosequencing
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DOI：
10.1007/s00253-013-5211-4
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发表时间：
2014-03-01
影响因子：
5
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
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作者：
Lu, Xiao-Ming;Lu, Peng-Zhen;Zhang, Hui
采用基于焦磷酸测序的分析方法对用不含添加剂的绿色饲料饲养的仔猪（GSPM）和成年猪（GBPM）以及用含添加剂的合成饲料饲养的仔猪（ASPM）和成年猪（ABPM）粪便样品中的细菌群落进行了表征和比较。对于每个样品，分别选择5，010个有效序列并用于进行细菌组成分析。在GBPM、GSPM、ABPM和ASPM中，以5%的距离截止值分别获得了总共1，489、1，327、941和883个操作分类单位。GBPM中细菌类群丰富度最高，ASPM中细菌类群丰富度最低。四个样品中最占优势的类群是类杆菌属。GBPM中约41%的序列属于梭菌目.在4份样品中检出弯曲杆菌属、梭菌属和链球菌属的致病菌。粪便中细菌的多样性和丰度因仔猪、成年猪和饲料而异。在4个样品中，绿色饲料饲养的成年猪粪便中细菌多样性较高，但潜在致病菌属较少，更适合资源化利用。本研究也为绿色饲料养猪的合理性提供了依据。
Pyrosequencing-based analysis was used to characterize and compare bacterial communities in manure samples of piglets (GSPM) and adult pigs (GBPM) bred with green feeds without additives, and piglets (ASPM) and adult pigs (ABPM) bred with synthetic feeds containing additives. For each sample, 5,010 effective sequences were selected and utilized to do the bacterial composition analysis, respectively. In total, 1,489, 1,327, 941, and 883 operational taxonomic units were obtained at 5 % distance cutoff in GBPM, GSPM, ABPM, and ASPM, respectively. Bacterial phylotype richness in GBPM was higher than the other samples, and ASPM had the least richness. The most dominant class in the four samples is Bacteroidia. Approximately 41 % sequences in GBPM were affiliated with the Clostridiales order. Campylobacter, Clostridium and Streptococcus genera containing pathogenic bacteria were detected in the four samples. Bacterial diversity and abundance in swine manures varied with piglets, adult pigs, and feeds. In the four samples, higher bacterial diversity but less potentially pathogenic bacterial genera in manures of adult pigs bred with green feeds were found, which indicated that those manures were more suitable for resource utilization. This study also provides evidence for the reasonableness of pig farming with green feeds.