SPLICED STRUCTURES OF ADENOVIRUS-2 FIBER MESSAGE AND OTHER LATE MESSENGER-RNAS
SPLICED STRUCTURES OF ADENOVIRUS-2 FIBER MESSAGE AND OTHER LATE MESSENGER-RNAS
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DOI：
10.1016/0092-8674(78)90019-3
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
BROKER, TR
中科院分区：
文献类型：
--
作者：
CHOW, LT;BROKER, TR
所有晚期腺病毒 2 [Ad2] mRNA（也许除了肽 IX 之外）都是复合分子，其序列源自基因组的至少 2-5 个或更多独立部分，如通过与单链或双链 DNA 杂交的多核糖体 RNA 的 EM 分析所确定的。先前报道，8种不同的向右转录的mRNA在其5''末端含有源自坐标16.6、19.6和26.6的共同3部分前导序列，并且第9个mRNA由直接与9.7-11.0的序列连接的4.6-6.1的序列组成。另外四种 RNA 物种也具有相同的三方前导序列，其 5'' 末端位于坐标 29.1、30.5、33.9 和 42.8 处。从29.1-39.0延伸的RNA转录覆盖了大部分或全部病毒相关(VA)RNA基因(29.0-30.1)。晚期RNA可分为几个转录本家族，其中2或3个mRNA具有相同的3''末端和3''近端序列，但5''末端的长度显着不同，因此前导序列附着到信息主体的坐标也不同。从坐标 29 到 50 之间的 Ad2 染色体转录的 RNA 种类数量超过了已识别的 Ad2 蛋白的数量。因此，长度相差百分之几的几对消息可能编码相同的蛋白质或具有前体-产物关系。纤维蛋白的多核糖体 mRNA 亚群，即右侧转录单位中最远端的基因 (86.3-91.2)，除了常见的三联前导序列之外，还具有第四个前导组件 y (78.6-79.1)。频率较低的纤维 RNA 种类具有第 5 个 x (76.9-77.3) 和/或第 6 个 z (84.7-85.1) 前导片段。具有 x、y 或 z 片段的转录本可能是加工中间体，并且可以将这些物种排列成阵列，暗示纤维 mRNA 成熟的多种途径。晚期从坐标15.0和11.0之间的L链转录的mRNA具有单个组分前导序列，其位于距坐标16.1-15.7的基因主体200个碱基处。该前导序列起源于非常接近 R 链信息的第一个前导成分 (16.5-16.6) 的位点，表明存在转录分歧的相邻 L 和 R 链启动子。总而言之，这些结果强调了后期 Ad2 mRNA 合成过程中剪接模式的多样性和重组序列的多样性。
All late adenovirus 2 [Ad2] mRNA, except perhaps that for peptide IX, are composite molecules with sequences derived from at least 2-5 or more separate portions of the genome, as determined by EM analyses of polysomal RNA hybridized to single- or double-stranded DNA. It was previously reported that 8 different rightward-transcribed mRNA contain, at their 5'' ends, a common 3-part leader sequence derived from coordinates 16.6, 19.6 and 26.6, and a 9th mRNA consists of sequences from 4.6-6.1 linked directly to those from 9.7-11.0. Four additional RNA species also have the same tripartite leader joined to messages with 5'' ends located at coordinates at 29.1, 30.5, 33.9 and 42.8. The RNA transcirpt extending from 29.1-39.0 covers most or all of the viral associated (VA) RNA genes (29.0-30.1). The late RNA can be grouped into several families of transcripts in which 2 or 3 mRNA have the same 3'' end and 3'' proximal sequences, but differ substantially in length at the 5'' end and, therefore, the coordinate at which the leader sequences are attached to the main body of the message. The number of RNA species transcribed from the Ad2 chromosome between coordinates 29 and 50 exceeds the number of identified Ad2 proteins. Several pairs of messages differing in length by a few percent may thus encode the same protein or have a precursor-product relationship. A subpopulation of the polysomal mRNA for the fiber protein, the most distal gene (86.3-91.2) in the rightward transcription units, has a 4th leader component y (78.6-79.1) in addition to the common tripartite leader sequences. Less frequent fiber RNA species have 5th x (76.9-77.3) and/or 6th z (84.7-85.1) leader segments. Trancripts with x,y or z segments might be processing intermediates, and it is possible to arrange these species into an array suggestive of multiple pathways for the maturation of fiber mRNA. mRNA transcribed at late times from the L strand between coordinates 15.0 and 11.0 has a single component leader located 200 bases from the main body of the gene from coordinate 16.1-15.7. This leader originates from a site very close to the 1st leader component (16.5-16.6) of the R strand messages and suggests the presence of adjacent L and R strand promoters from which transcription diverges. Taken together, these results emphasize the diversity of splicing patterns and the variety of recombined sequences generated during the synthesis of late Ad2 mRNA.