New potentiometric microbial biosensor for ethanol determination in alcoholic beverages
New potentiometric microbial biosensor for ethanol determination in alcoholic beverages
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DOI：
10.1016/j.aca.2003.12.048
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发表时间：
2004-06
影响因子：
6.2
通讯作者：
L. Rotariu;C. Bala;V. Magearu
中科院分区：
文献类型：
--
作者：
L. Rotariu;C. Bala;V. Magearu
以固定化酵母细胞（Saccharomyces ellipsoideus）为载体，利用电位型氧电极成功地制备了一种响应范围较宽的微生物传感器。对乙醇的反应需要大约。稳态法为7 min，动力学法为2 min。线性校准超过二十年的乙醇浓度。在非挥发性化合物的存在下，乙醇的选择性测定实现使用第二PTFE膜覆盖的生物催化层。
A potentiometric oxygen electrode was successfully used to produce a microbial biosensors with an extended response range, based on immobilised yeast cells (Saccharomyces ellipsoideus). The response for ethanol takes ca. 7min for the steady-state method and 2 min for kinetic measurements. A linear calibration achieved over more than two decades of ethanol concentration. Selective determination of ethanol in the presence of non-volatile compounds was achieved using a second PTFE membrane to cover the biocatalytic layer.