In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis
In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis
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结核分枝杆菌 Rv3425 的新型细胞毒性 T 淋巴细胞表位的体外和体内鉴定
DOI：
10.1111/j.1348-0421.2012.00470.x
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发表时间：
2012-08-01
影响因子：
2.6
通讯作者：
Gao, Yan-feng
中科院分区：
文献类型：
--
作者：
Chen, Fei;Zhai, Ming-xia;Gao, Yan-feng
新的细胞毒性T淋巴细胞（CTL）表位的鉴定对于分析CD8+ T细胞在结核分枝杆菌感染中的作用以及肽疫苗的开发具有重要意义。在这项研究中，从差异11编码抗原Rv3425区鉴定了一个新的CTL表位。用逆转免疫学方法预测抗原表位。Rv3425‐p118 （LIASNVAGV）对HLA‐A*0201分子具有较强的结合亲和力和稳定性。在健康供体（HLA - A*02+纯化蛋白衍生物+）中，受此肽刺激的外周血单核细胞能够释放干扰素γ。在体外和体内细胞毒性实验中，Rv3425‐p118诱导ctl特异性裂解靶细胞。因此，该表位可为设计抗结核分枝杆菌疫苗提供亚基成分。
The identification of novel cytotoxic T lymphocyte (CTL) epitopes is important to analysis of the involvement of CD8+ T cells in Mycobacterium tuberculosis infection as well as to the development of peptide vaccines. In this study, a novel CTL epitope from region of difference 11 encoded antigen Rv3425 was identified. Epitopes were predicted by the reversal immunology approach. Rv3425‐p118 (LIASNVAGV) was identified as having relatively strong binding affinity and stability towards the HLA‐A*0201 molecule. Peripheral blood mononuclear cells pulsed by this peptide were able to release interferon‐γ in healthy donors (HLA‐A*02+ purified protein derivative+). In cytotoxicity assays in vitro and in vivo, Rv3425‐p118 induced CTLs to specifically lyse the target cells. Therefore, this epitope could provide a subunit component for designing vaccines against Mycobacterium tuberculosis.