Inositol 1,4,5-Trisphosphate-Gated Calcium Transport through Plasma Membranes in Nerve Terminals
Inositol 1,4,5-Trisphosphate-Gated Calcium Transport through Plasma Membranes in Nerve Terminals
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肌醇 1,4,5-三磷酸门控钙通过神经末梢质膜的转运
DOI：
10.1523/jneurosci.16-09-02891.1996
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Satoh
中科院分区：
文献类型：
--
作者：
H. Ueda;S. Tamura;N. Fukushima;T. Katada;M. Ui;M. Satoh
我们开发了新的生化方法来证明突触前质膜 (SPM) 中肌醇 1,4,5-三磷酸 (InsP3) 门控钙通道的存在及其参与突触前受体介导的 Ca2+ 流入神经末梢。在使用预载45Ca2+的SPM囊泡的灌注实验中，InsP3引发45Ca2+以饱和方式释放到灌注液中。重新密封的 SPM 囊泡中 InsP3 诱发的 45Ca2+ 释放比使用任何其他亚细胞组分重新密封的囊泡释放的效果更强。在这里，我们还报告了通过使用用纯化的 Gi1 重建的这种重新密封的囊泡，InsP3 门控机制参与突触前受体介导的 Ca2+ 流入突触体（神经末梢）。
We developed new biochemical approaches to demonstrate the presence of inositol 1,4,5-trisphosphate (InsP3)-gated calcium channels in presynaptic plasma membranes (SPM) and their involvement in the presynaptic receptor-mediated Ca2+ influx into nerve terminals. In perfusion experiments using SPM vesicles preloaded with45Ca2+, InsP3 elicited the release of45Ca2+ into perfusates in a saturable manner. The InsP3-evoked45Ca2+ release from resealed SPM vesicles was more potent than that from resealed vesicles using any other subcellular fractions. Here we also report the involvement of InsP3-gated mechanisms in the presynaptic receptor-mediated Ca2+ influx into synaptosomes (nerve terminals) by use of such resealed vesicles reconstituted with purified Gi1.