Spatially distinct mitochondrial populations exhibit different mitofilin levels
Spatially distinct mitochondrial populations exhibit different mitofilin levels
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DOI：
10.1002/cbf.2811
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发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Amado, Francisco
中科院分区：
文献类型：
--
作者：
Ferreira, Rita M. P.;Vitorino, Rui;Amado, Francisco
肌膜下（SS）和肌原纤维间（IMF）的线粒体表现出独特的生化和功能特性，然而，它们与控制横纹肌组织中线粒体形态和功能的结构膜蛋白的关系从未报道过。在从大鼠心脏和腓肠肌分离的IMF和SS线粒体中，我们分析了丝裂素的表达水平，丝裂素是一种参与细胞器结构维持的线粒体相关蛋白。与SS线粒体相比，在IMF中检测到的丝裂素的量在统计学上显著更高，在心脏组织中为37倍，在腓肠肌中为3.8倍，以及这些线粒体群体的特定能量需求突出了丝裂素在氧化磷酸化功能和横纹肌中线粒体可塑性中的重要性。IMF和SS之间的mitofilin的差异表达水平也表明，这种蛋白质可以被用作一个特定的分子标记，以比较区分空间上遥远的线粒体种群。版权所有（C）2012约翰威利父子有限公司
Subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria exhibit unique biochemical and functional properties; however, their association with structural membrane proteins that control mitochondrial morphology and functionality in striated muscle tissue was never reported. In IMF and SS mitochondria isolated from rat heart and gastrocnemius muscle, we analysed the expression levels of mitofilin, a mitochondria-associated protein involved in organelle structure maintenance. The statistically significant higher amounts of mitofilin detected in IMF compared with SS mitochondria, 37-fold in cardiac tissue and 3.8-fold in gastrocnemius, together with the specific energetic requirements of these mitochondrial populations highlight the importance of mitofilin in oxidative phosphorylation functionality and in mitochondrial plasticity in striated muscle. The differential expression levels of mitofilin between IMF and SS also suggest that this protein can be used as a specific molecular marker to comparatively discriminate spatially distant mitochondrial populations. Copyright (C) 2012 John Wiley & Sons, Ltd.