EVALUATION OF AFFIRM VP MICROBIAL IDENTIFICATION TEST FOR GARDNERELLA-VAGINALIS AND TRICHOMONAS-VAGINALIS
EVALUATION OF AFFIRM VP MICROBIAL IDENTIFICATION TEST FOR GARDNERELLA-VAGINALIS AND TRICHOMONAS-VAGINALIS
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DOI：
10.1128/jcm.32.1.148-152.1994
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发表时间：
1994-01-01
影响因子：
9.4
通讯作者：
HILLIER, SL
中科院分区：
文献类型：
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作者：
BRISELDEN, AM;HILLIER, SL
商业系统(确认VP微生物鉴定测试；MicroProbe Corp.)对176名因生殖器疾病就诊性传播疾病诊所的连续妇女进行了阴道病原体检测的评估。阴道拭子标本用于阴道加德纳氏菌和阴道毛滴虫的培养，制备阴道涂片用于革兰氏染色鉴定，并进行湿法检测。用另外一根拭子来评估30分钟的非同位素寡核苷酸探针测试。在73例阴道毛滴虫培养中检出阴道毛滴虫5×10(5)cfu/m l的69例(95%)，阴道毛滴虫<5×10(5)cfu/m l的47例中检出20例(43%)。阴道毛滴虫5×10(5)CFU/ml，63例有线索细胞的阴道标本中检出阴道毛滴虫57例(90%)，113例无线索细胞的阴道标本中检出阴道毛滴虫3例(3%)，提示阴道毛滴虫确证探针可作为阴道毛滴虫湿法检测的替代方法。12份湿法阳性标本和15份培养阳性标本中有12份阴道毛滴虫探头阳性。与培养和/或湿载阴道毛滴虫相比，确认VP试验没有假阳性和3例假阴性。确证VP微生物鉴定系统是一种快速、客观和自动化的检测方法，用于检测阴道毛滴虫和临床显著水平的阴道毛滴虫，可与线索细胞湿法检测相媲美，在检测滴虫方面优于湿法检测。
A commercial system (Affirm VP Microbial Identification Test; MicroProbe Corp.) for detection of vaginal pathogens was evaluated with 176 consecutive women attending a sexually transmitted disease clinic for genital complaints. Vaginal swab specimens were used for culture of Gardnerella vaginalis and Trichomonas vaginalis, preparation of a vaginal smear for Gram stain interpretation, and wet mount evaluation. An additional swab was used to evaluate the 30-min nonisotopic oligonucleotide probe test. The automated probe system detected G. vaginalis in 69 (95%) of 73 women having >5 x 10(5) CFU of G. vaginalis per ml by culture, and 20 (43%) of 47 specimens with less-than-or-equal-to 5 x 10(5) CFU of G. vaginalis per ml. There were three false positives and four false negatives for the Affirm VP test compared with >5 x 10(5) CFU of G. vaginalis per ml. The probe system detected G. vaginalis in 57 (90%) of 63 vaginal specimens from women having clue cells on wet mount examination, and in only 3 (3%) of 113 women without clue cells, suggesting that the Affirm probe for G. vaginalis could be used as a surrogate for wet mount examination for clue cells. The T. vaginalis probe was positive for 12 of 12 specimens positive by wet mount and 12 of 15 specimens positive by culture. There were no false positives and three false negatives for the Affirm VP test compared with culture and/or wet mount for T. vaginalis. The Affirm VP Microbial Identification System is a rapid, objective, and automated test for the detection of T. vaginalis and clinically significant levels of G. vaginalis that is comparable to wet mount examination for clue cells and is superior to wet mount examination for the detection of trichomonads.