Wolbachia-induced cytoplasmic incompatibility in Japanese populations of Tetranychus urticae (Acari: Tetranychidae)
Wolbachia-induced cytoplasmic incompatibility in Japanese populations of Tetranychus urticae (Acari: Tetranychidae)
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DOI：
10.1007/s10493-007-9072-3
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发表时间：
2007-05-01
影响因子：
2.2
通讯作者：
Kitashima, Yasuki
中科院分区：
文献类型：
--
作者：
Gotoh, Tetsuo;Sugasawa, Jun;Kitashima, Yasuki
沃尔巴克氏体属的细胞内细菌（α变形菌门）在许多节肢动物物种（包括蜘蛛螨）中诱导细胞质不相容性（CI），但并非所有沃尔巴克氏体都会引起CI。在叶螨CI变得明显的卵孵化率降低和较低的女儿：儿子的比例：CI在一般的单倍体生物体预计将产生全男性后代或男性偏见的性别比例没有任何死亡的鸡蛋。在之前对日本二斑叶螨种群的一项研究中，测试的三分之二的绿色种群感染了非CI沃尔巴克氏体菌株，而六个红色种群中没有一个携带沃尔巴克氏体。同时对T.尽管日本的荨麻疹仍是零星的，但我们在从日本广泛岛屿收集的30个绿型种群和29个红型种群中检查了沃尔巴克氏体感染。对于感染沃尔巴克氏体的种群，我们测试了沃尔巴克氏体对繁殖性状的影响，并确定了不同沃尔巴克氏体菌株的系统发育关系。除了一个绿色形式的人口感染沃尔巴克氏体和所有菌株属于亚组Ori时，wsp基因被用来确定不同菌株的沃尔巴克氏体的系统发育关系。29个红型种群中有6个携带沃尔巴克氏体，感染的菌株属于Ori和Bugs亚群。29个感染的绿型种群中有24个和6个感染的红型种群中有5个诱导了宿主间的CI。因此，CI-沃尔巴克氏体菌株在日本广泛分布，在CI-沃尔巴克氏体中没有观察到地理趋势。虽然三个红色形式的人口窝藏其他细胞内的细菌Cardinium，他们不影响宿主繁殖。
Intracellular bacteria of the genus Wolbachia (alpha Proteobacteria) induce cytoplasmic incompatibility (CI) in many arthropod species, including spider mites, but not all Wolbachia cause CI. In spider mites CI becomes apparent by a reduced egg hatchability and a lower daughter:son ratio: CI in haplodiploid organisms in general was expected to produce all-male offspring or a male-biased sex ratio without any death of eggs. In a previous study of Japanese populations of Tetranychus urticae, two out of three green-form populations tested were infected with non-CI Wolbachia strains, whereas none of six red-form populations harbored Wolbachia. As the survey of Wolbachia infection in T. urticae is still fragmentary in Japan, we checked Wolbachia infection in thirty green-form populations and 29 red-form populations collected from a wide range of Japanese islands. For Wolbachia-infected populations, we tested the effects of Wolbachia on the reproductive traits and determined the phylogenetic relationships of the different strains of Wolbachia. All but one green-form populations were infected with Wolbachia and all strains belonged to the subgroup Ori when the wsp gene was used to determine the phylogenetic relationships of different strains of Wolbachia. Six out of 29 red-form populations harbored Wolbachia and the infected strains belonged to the subgroups Ori and Bugs. Twenty-four of 29 infected green-form populations and five of six infected red-form populations induced CI among the hosts. Thus, CI-Wolbachia strains are widespread in Japan, and no geographical trend was observed in the CI-Wolbachia. Although three red-form populations harbored other intracellular bacteria Cardinium, they did not affect host reproduction.