Isolation and functional characterization of PgTIP1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng
Isolation and functional characterization of PgTIP1 a hormone-autotrophic cells-specific tonoplast aquaporin in ginseng
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DOI：
10.1093/jxb/erl255
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发表时间：
2007-01-01
影响因子：
6.9
通讯作者：
Cai, Weiming
中科院分区：
文献类型：
--
作者：
Lin, Wuling;Peng, Yanhui;Cai, Weiming
采用抑制性消减杂交技术筛选激素自养和激素依赖的人参愈伤组织之间的差异表达基因。在激素自养的人参细胞中，液泡膜固有蛋白基因(PgTiP1)特异性地高表达，在细胞分裂素诱导下略有上调，而在生长素处理下显著下调。PgTIP1编码250个氨基酸的多肽，与植物中的液泡膜水通道蛋白在序列和结构上具有相似性。PgTIP1在非洲爪哇卵母细胞中的表达表明其具有水通道活性。当PgTIP1在拟南芥中过表达时，它极大地改变了植物的营养和生殖生长发育。与野生型相比，高表达PgTIP1的拟南芥植株的种子大小和种子质量显着增加，生长速率显着提高。此外，高表达PgTIP1的拟南芥种子的脂肪酸含量是野生型对照的1.85倍。这些结果表明，PgTIP1在植物细胞的生长发育中具有重要的功能。
The suppression subtractive hybridization technique was used to identify differentially expressed genes between hormone-autotrophic and hormone-dependent Panax ginseng callus lines. A tonoplast intrinsic protein cDNA (PgTiP1) was found to be highly and specifically expressed in hormone-autotrophic ginseng cells, which was slightly up-regulated by cytokinin while significantly down-regulated when treated with auxin. PgTIP1 encodes a polypeptide of 250 amino acids which shows sequence and structure similarity with tonoplast aquaporins in plants. The water channel activity of PgTIP1 was demonstrated by its expression in Xenopus laevis oocytes. When over-expressed in Arabidopsis thaliana, PgTIP1 substantially altered the plant's vegetative and reproductive growth and development. Arabidopsis plants over-expressing PgTIP1 showed significantly enhanced seed size and seed mass plus greatly increased growth rate compared with those of the wild type. Moreover, the seeds from PgTIP1 over-expressing Arabidopsis had 1.85-fold higher fatty acid content than the wild-type control. These results demonstrate a significant function of PgTIP1 in the growth and development of plant cells.