Effect of submicron and nano-iron oxide particles on pulmonary immunity in mice
Effect of submicron and nano-iron oxide particles on pulmonary immunity in mice
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DOI：
10.1016/j.toxlet.2012.02.004
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发表时间：
2012-05-05
影响因子：
3.5
通讯作者：
Goutet, Michele
中科院分区：
文献类型：
--
作者：
Ban, Masarin;Langonne, Isabelle;Goutet, Michele
由于纳米技术的进步，在工作场所接触颗粒化合物已变得不可避免。评估其对健康的毒性是一个重要的职业安全问题。这项研究在小鼠中进行，旨在研究亚微米和纳米氧化铁颗粒对肺部免疫防御的毒理学影响。为此，我们首次探索了肺相关淋巴结的炎症和免疫反应。对于每种颗粒类型，小鼠接受不同浓度（250、375或500μg/小鼠）的单次气管内滴注，或者以每次500μg/小鼠的四次重复滴注。分别在颗粒暴露后 1、2 和 6 天评估细胞因子的产生、炎症和先天免疫反应以及体液免疫反应。两种类型的颗粒都会诱发肺部炎症，并与淋巴结细胞培养物中细胞因子产生增加相关，并降低针对绵羊红细胞的肺部免疫反应。粒子不会改变自然杀伤活性。与单次滴注相比，重复滴注导致支气管肺泡灌洗液和肺实质中炎症细胞数量减少。此外，单次滴注模型表明，在相同剂量下，纳米氧化铁颗粒比亚微米颗粒产生更高水平的炎症和免疫抑制。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
Due to advances in nanotechnology, exposure to particle compounds in the workplace has become unavoidable. Assessment of their toxicity on health is an important occupational safety issue. This study was conducted in mice to investigate the toxicological effects of submicron and nano-iron oxide particles on pulmonary immune defences. In that purpose, we explored for the first time, inflammatory and immune responses in lung-associated lymph nodes. For each particle type, mice received either a single intratracheal instillation at different concentrations (250, 375, or 500 mu g/mouse) or four repeated instillations at 500 mu g/mouse each. Cytokine production, inflammatory and innate immune response, and humoral immune response were respectively assessed 1, 2, and 6 days after particle exposures. Both types of particles induced lung inflammation associated with increased cytokine productions in lymph node cell cultures and decreased pulmonary immune responses against sheep erythrocytes. Natural killer activity was not modified by particles. In comparison to single instillation, repeated instillations resulted in a reduction of inflammatory cell numbers in both bronchoalveolar lavages and pulmonary parenchyma. Moreover, the single instillation model demonstrated that, for a same dose, nano-iron oxide particles produced higher levels of inflammation and immunodepression than their submicron-sized counterparts. (C) 2012 Elsevier Ireland Ltd. All rights reserved.