Antibodies to distinct polypeptides of RNA polymerase I in sera from patients with rheumatic autoimmune disease.
Antibodies to distinct polypeptides of RNA polymerase I in sera from patients with rheumatic autoimmune disease.
复制标题
风湿性自身免疫性疾病患者血清中 RNA 聚合酶 I 不同多肽的抗体。
DOI：
10.1073/pnas.79.23.7499
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发表时间：
1982
影响因子：
11.1
通讯作者：
Jacob,ST
中科院分区：
文献类型：
--
作者：
Stetler,DA;Rose,KM;Wenger,ME;Berlin,CM;Jacob,ST
用固相放射免疫分析法筛选风湿性自身免疫性疾病患者血清中针对RNA聚合酶I的抗体。在所有系统性红斑狼疮或混合性结缔组织病患者以及78%的类风湿性关节炎患者的血清中均检测到显著水平的抗体。在健康受试者的血清中没有发现可检测到的抗RNA聚合酶I抗体。服用肼丙嗪的患者中，有三人出现了药物诱导的狼疮症状，几乎检测不到抗体水平。从含抗RNA聚合酶I抗体的血清中提取的免疫球蛋白，经放射免疫法测定，在体外可抑制RNA聚合酶I的活性。系统性红斑狼疮患者的血清含有针对MR 65,000的聚合酶I相关多肽以及MR 120,000或MR 25,000的多肽的免疫球蛋白，或两者兼而有之。类风湿性关节炎患者的血清只与MR 65,000的多肽反应。混合性结缔组织病患者血清中的抗体针对MR 42,000多肽或MR 65,000，42,000和25,000多肽的组合。这些数据表明，抗RNA聚合酶I抗体的产生可能是风湿性自身免疫性疾病患者的独特特征，并且针对RNA聚合酶I的特定多肽的抗体的产生可能指示特定的疾病类别。
Sera from patients with rheumatic autoimmune diseases were screened for antibodies directed against RNA polymerase I by using a solid-phase radioimmunoassay. Significant levels of the antibodies were detected in the sera of all patients with either systemic lupus erythematosus or mixed connective tissue disease and in 78% of the individuals with rheumatoid arthritis. No detectable anti-RNA polymerase I antibodies were found in the sera from healthy subjects. Individuals taking hydralazine, three of whom exhibited symptoms of drug-induced lupus, had barely detectable levels of the antibodies. Immunoglobulins obtained from sera containing anti-RNA polymerase I antibodies, as determined by the radioimmunoassay, could inhibit RNA polymerase I activity in vitro. Sera from patients with systemic lupus erythematosus contained immunoglobulins directed against the polymerase I-associated polypeptide of Mr 65,000 as well as against the polypeptides of Mr 120,000 or Mr 25,000, or both. Sera from individuals with rheumatoid arthritis reacted with the polypeptide of Mr 65,000 only. The antibodies in the sera of patients with mixed connective tissue disease were directed against the Mr 42,000 polypeptide or a combination of the Mr 65,000, 42,000, and 25,000 polypeptides. These data suggest that the production of anti-RNA polymerase I antibodies may be a unique characteristic of individuals with rheumatic autoimmune diseases and that the production of antibodies against specific polypeptides of RNA polymerase I may be indicative of the particular class of disease.