Recombinant hepatitis E virus genomes infectious for primates: Importance of capping and discovery of a cis-reactive element
Recombinant hepatitis E virus genomes infectious for primates: Importance of capping and discovery of a cis-reactive element
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DOI：
10.1073/pnas.251555098
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发表时间：
2001-12-18
影响因子：
11.1
通讯作者：
Purcell, RH
中科院分区：
文献类型：
--
作者：
Emerson, SU;Zhang, M;Purcell, RH
戊型肝炎病毒重组基因组体外转录的两个cDNA克隆不同的两个核苷酸的黑猩猩感染。然而，一个cDNA克隆编码的病毒在黑猩猩中被减毒，不能感染恒河猴。第二个cDNA克隆编码的病毒感染了黑猩猩和恒河猴，并在两者中引起急性肝炎。区分两个克隆的一个突变鉴定了一个顺式反应元件，该元件似乎与衣壳基因的3'端和3'非编码区的一部分重叠。RNA转录物的加帽对于感染性是必不可少的。
Hepatitis E virus recombinant genomes transcribed in vitro from two cDNA clones differing by two nucleotides were infectious for chimpanzees. However, one cDNA clone encoded a virus that was attenuated for chimpanzees and unable to infect rhesus monkeys. The second cDNA clone encoded a virus that infected both chimpanzees and rhesus monkeys and caused acute hepatitis in both. One mutation differentiating the two clones identified a cis-reactive element that appeared to overlap the 3' end of the capsid gene and part of the 3' noncoding region. Capping of the RNA transcripts was essential for infectivity.