Autologous synovial fluid enhances migration of mesenchymal stem cells from synovium of osteoarthritis patients in tissue culture system
Autologous synovial fluid enhances migration of mesenchymal stem cells from synovium of osteoarthritis patients in tissue culture system
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DOI：
10.1002/jor.20659
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发表时间：
2008-10-01
影响因子：
2.8
通讯作者：
Sekiya, Ichiro
中科院分区：
文献类型：
--
作者：
Zhang, Sheng;Muneta, Takeshi;Sekiya, Ichiro
骨关节炎膝关节滑液中含有间充质干细胞。关节滑液中MSC的可能储存库之一是滑膜组织，并且关节滑液可以诱导MSC动员到骨关节炎患者的关节滑液中。在这里，我们研究了滑液是否在组织培养系统中扩增了滑液MSC。在全膝关节置换术期间从骨关节炎患者获得人滑膜和滑液。在组织培养系统中，自体滑液扩增的滑膜细胞在统计学上高于α MEM + FBS，并且在所有11名供体中向α MEM + FBS中添加TGF β 3将扩增增加至相似水平。向滑液中加入核心蛋白聚糖或抗TGF β中和抗体部分抑制了滑膜细胞扩增。在细胞培养试验中，滑液增殖的滑膜细胞少于α MEM + FBS。滑膜液中扩增的滑膜细胞保留了多能性，并显示出与MSC相似的表面标志物。我们证明了自体滑液通过促进细胞迁移增强了骨关节炎患者滑膜组织培养中MSC的扩增。这种作用部分受到TGF β的影响。(C)2008骨科研究学会。由威利期刊公司出版
Synovial fluid from osteoarthritic knee contains mesenchymal stem cells (MSCs). One of the possible reservoirs of MSCs in synovial fluid is synovial tissue, and synovial fluid may induce mobilization of MSCs into synovial fluid in osteoarthritis patients. Here, we investigated whether synovial fluid expanded synovial MSCs in a tissue culture system. Human synovium and synovial fluid were obtained from osteoarthritis patients during total knee arthroplasties. In the tissue culture system, autologous synovial fluid expanded synovial cells statistically higher than alpha MEM + FBS, and the addition of TGF beta 3 to alpha MEM + FBS increased expansion to a similar level in all 11 donors. The addition of decorin or anti-TGF beta neutralizing antibody to synovial fluid partially inhibited synovial cell expansion. In cell culture assay, synovial fluid proliferated synovial cells fewer than alpha MEM + FBS. The expanded synovial cells in synovial fluid retained multipotentiality and showed surface markers similar to those of MSCs. We demonstrated that autologous synovial fluid enhanced expansion of MSCs in tissue culture of synovium from osteoarthritis patients by promoting cell migration. This effect was partially affected by TGF beta. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.