Renal denervation in the rat: analysis of glomerular and proximal tubular function.
Renal denervation in the rat: analysis of glomerular and proximal tubular function.
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大鼠肾去神经支配：肾小球和近端肾小管功能分析。
DOI：
10.1152/ajprenal.1983.244.1.f70
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Blantz,RC
中科院分区：
文献类型：
--
作者：
Pelayo,JC;Ziegler,MG;Jose,PA;Blantz,RC
我们研究了1）单个肾单位滤过率（SNGFR）和肾小球超滤决定因素的潜在变化，以及2）肾小管周围毛细血管（PTC）“物理因素”的变化，这些因素可能导致急性肾去神经支配（DNx）后近端肾小管重吸收（APR）的变化。在具有DNx或假手术（sham）的等容性Munich-Wistar大鼠中获得微穿刺测量。DNx组肾组织匀浆中去甲肾上腺素含量明显低于假肾组（P <0.001）。平均动脉血压、红细胞压积、全肾GFR和尿流率在假手术组和DNx组之间没有差异。DNx组的绝对尿钠排泄量是假手术组的3倍（P <0.01）。SNGFR及其决定因素在两种实验条件下无统计学差异。DNx组的APR显著降低约25%（P <0.02）。APR的这种降低并不伴随着肾小管周围毛细血管和肾间质压力的显著方向性变化以及作用于PTC近端肾小管上皮的被动驱动力。这些数据表明，肾神经支配的消除不改变SNGFR或其决定因素，并表明去神经支配对APR的影响是一个主要的上皮事件，发生独立的肾间质压力和管周压力和静水压力的变化。
We examined 1) the potential modifications in both single nephron filtration rate (SNGFR) and the determinants of glomerular ultrafiltration, and 2) the alterations in peritubular capillary (PTC) "physical factors" that may contribute to changes in proximal tubular reabsorption (APR) after acute renal denervation (DNx). Micropuncture measurements were obtained in euvolemic Munich-Wistar rats with DNx or sham operation (sham). The content of norepinephrine in renal tissue homogenates was markedly reduced in DNx kidneys compared with sham kidneys (P less than 0.001). Mean arterial blood pressure, hematocrit, whole kidney GFR, and urinary flow rate were not different between the sham and DNx groups. Absolute urinary sodium excretion was 3 times greater in the DNx than in the sham group (P less than 0.01). SNGFR and its determinants were not statistically different in the two experimental conditions. APR was significantly reduced by approximately 25% in DNx (P less than 0.02). This reduction in APR was not accompanied by significant directional changes in peritubular capillary and renal interstitial pressures and the passive driving forces acting across the PTC-proximal tubular epithelium. These data demonstrate that elimination of renal innervation does not alter SNGFR or its determinants and suggest that the effect of denervation on APR is a primary epithelial event that occurs independent of changes in renal interstitial pressure and peritubular oncotic and hydrostatic pressures.