LSECs express functional NOD1 receptors: A role for NOD1 in LSEC maturation-induced T cell immunity in vitro
LSECs express functional NOD1 receptors: A role for NOD1 in LSEC maturation-induced T cell immunity in vitro
复制标题
LSEC 表达功能性 NOD1 受体：NOD1 在 LSEC 成熟诱导的体外 T 细胞免疫中的作用
DOI：
10.1016/j.molimm.2018.06.002
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发表时间：
2018-09-01
影响因子：
3.6
通讯作者：
Yang, Dongliang
中科院分区：
文献类型：
--
作者：
Huang, Shunmei;Wu, Jun;Yang, Dongliang
Liver sinusoidal endothelial cells (LSECs) are organ resident APCs capable of antigen presentation and subsequent tolerization of T cells under physiological conditions. In this study, we investigated whether LSEC pretreatment with NOD-like receptor (NLR) agonists can switch the cells from a tolerogenic to an immunogenic state and promote the development of T cell immunity. LSECs constitutively express NOD1, NOD2 and RIPK2. Stimulation of LSECs with DAP induced the activation of NF-kappa B and MAP kinases and upregulated the expression of chemokines (CXCL2/9, CCL2/7/8) and cytokines (IFN-gamma, TNF-alpha and IL-2). Pretreatment of LSECs with DAP induced significantly increased IFN-gamma and IL-2-production by HBV-stimulated CD8(+) T cells primed by DAPtreated LSECs. Consistently, a significant reduction in the HBV DNA and HBsAg level occurred in mice receiving T cells primed by DAP-treated LSECs. MDP stimulation had no impact on LSECs or HBV-stimulated CD8(+) T cells primed with MDP-treated LSECs except for the upregulation of PD-L1. DAP stimulation in vitro could promote LSEC maturation and activate HBV-specific T cell responses. These results are of particular relevance for the regulation of the local innate immune response against HBV infections.
