ADSORPTIVE ENDOCYTOSIS OF SEMLIKI FOREST VIRUS
ADSORPTIVE ENDOCYTOSIS OF SEMLIKI FOREST VIRUS
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DOI：
10.1016/0022-2836(80)90281-8
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发表时间：
1980-01-01
影响因子：
5.6
通讯作者：
HELENIUS, A
中科院分区：
文献类型：
--
作者：
MARSH, M;HELENIUS, A
研究了塞姆利基森林病毒（SFV）在[幼鼠肾]BHK-21细胞中的内吞噬作用，其多重度在1到4倍之间变化。105病毒/细胞。摄取需要附着在细胞表面，非常迅速（半衰期10-35分钟），发生在包被的囊泡中，在< 15度的温度下被抑制。C，它不会提高细胞的液相摄取。利用colcolid， cytochalasin B，叠氮化钠，二硝基苯酚，2-脱氧-d -葡萄糖和溶酶体性弱碱的抑制剂研究表明，摄取在很大程度上独立于细胞骨架和溶酶体功能，但部分依赖于氧化磷酸化。生化和形态学结果表明，SFV的吸收是通过吸附内吞作用发生的，其方式与受体介导的血清低密度脂蛋白和其他生理上重要的细胞外蛋白的内吞作用非常相似。因此，SFV似乎利用兼性细胞过程进入细胞。SFV的吸收被用来研究吸附内吞过程的定量方面。在病毒向细胞高增殖时，每个细胞每分钟可内化1500-3000个病毒颗粒。电镜显示平均1.3个病毒/含病毒包膜囊泡。
Endocytosis of Semliki Forest virus [SFV] into [baby hamster kidney] BHK-21 cells was studied at multiplicities varying between 1 virus and 4 .times. 105 viruses/cell. Uptake requires attachment to the cell surface, it is very rapid (half-life 10-35 min), it occurs in coated vesicles, it is inhibited at temperatures < 15.degree. C and it does not elevate the fluid-phase uptake of the cells. Inhibitor studies using colcemid, cytochalasin B, sodium azide, dinitrophenol, 2-deoxy-D-glucose and lysosomotropic weak bases show that the uptake is largely independent of cytoskeletal and lysosomal function, but partially dependent on oxidative phosphorylation. Together the biochemical and morphological results indicate that SFV uptake occurs by adsorptive endocytosis in a manner very similar to the receptor-mediated endocytosis of serum low-density lipoprotein and other physiologically important extracellular proteins. SFV thus appears to exploit a facultative cellular process in gaining entry into cells. The uptake of SFV was used to study quantitative aspects of the adsorptive endocytosis process. At high multiplicites of viruses to cells, 1500-3000 virus particles can be internalized/min per cell. EM indicated an average of 1.3 viruses/virus-containing coated vesicle.