Inorganic pyrophosphate concentrations in the synovial fluid of arthritic patients.
Inorganic pyrophosphate concentrations in the synovial fluid of arthritic patients.
复制标题
关节炎患者滑液中无机焦磷酸盐的浓度。
DOI：
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发表时间：
1971
期刊：
影响因子：
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通讯作者：
E. Paloyan
中科院分区：
文献类型：
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作者：
D. Mccarty;S. Solomon;M. Warnock;E. Paloyan
摘要 描述了用于测量滑液中无机焦磷酸盐 (PPi) 的放射性同位素稀释技术。基于重复测量的其精度为每 100 毫升 ±4 微克。除假痛风外的关节炎患者的 23 份关节液中，有 20 份的 PPi 水平集中在平均值±标准差附近。 22±6.3； 3 种对照液显示出升高的值。在来自焦磷酸钙晶体沉积病（假痛风）患者的 18 份关节液中，15 份显示无机 PPi 水平大于 2 S.D.。从控制平均值来看，2 例处于临界值，1 例落在正常范围内。平均值±标准差该组的值为 100.4 ± 93。对照和假痛风患者关节液中的无机正磷酸盐、总钙和离子钙、针对对硝基苯磷酸盐和 β-甘油磷酸盐的碱性磷酸单酯酶活性以及碱性磷酸单酯酶同工酶没有显着差异。对二水焦磷酸钙混晶在蒸馏水、Tris 缓冲液、透析和厌氧处理的新鲜关节液和血浆等各种溶剂中的溶解度进行了初步研究。所达到的 PPi 水平与假痛风患者关节液中的水平处于同一数量级。镁离子和蛋白质溶解度增加；钙和正磷酸盐离子降低了它。目前的数据无法回答假性痛风液中的 PPi 水平是否代表体内饱和水平。
Abstract A radioisotope dilution technique for measurement of inorganic pyrophosphate (PPi) in synovial fluid is described. Its precision based on duplicate measurements is ±4 μg per 100 ml. PPi levels in 20 of 23 joint fluids from patients with arthritis other than pseudogout clustered about a mean ± S.D. of 22 ± 6.3; 3 control fluids showed elevated values. Of 18 joint fluids from patients with calcium pyrophosphate crystal deposition disease (pseudogout), 15 showed inorganic PPi levels that were greater than 2 S.D. from the control mean, 2 were borderline, and one fell in the normal range. The mean ± S.D. of the group was 100.4 ± 93. Inorganic orthophosphate, total and ionized calcium, alkaline phosphomonoesterase activity against both p-nitrophenylphosphate and β-glycerophosphate, and alkaline phosphomonoesterase isozymes were not significantly different in joint fluids from control and pseudogout patients. Preliminary studies on the solubility of calcium pyrophosphate dihydrate miscocrystals in various solvents such as distilled water, Tris buffer, dialyzed and anaerobically processed fresh joint fluid and plasma are presented. The levels of PPi attained were of the same order of magnitude as those found in joint fluids from pseudogout patients. Magnesium ions and protein increased solubility; calcium and orthophosphate ions decreased it. Whether or not the PPi levels found in pseudogout fluid represent saturation levels in vivo cannot be answered from the present data.