Screening 34 Peanut Introductions for Allergen Content Using ELISA
Screening 34 Peanut Introductions for Allergen Content Using ELISA
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DOI：
10.1080/09540100220145179
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发表时间：
2002-06
影响因子：
3
通讯作者：
H. Dodo;Damien Marsic;M. Callender;E. Cebert;O. Viquez
中科院分区：
文献类型：
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作者：
H. Dodo;Damien Marsic;M. Callender;E. Cebert;O. Viquez
花生是最易致敏的食物之一，意外摄入花生的报告正在增加。目前还没有关于商业化种植和用于食品和糖果工业的花生种质的过敏原含量的信息。本研究的目的是：（1）确定34个花生引进（PI）的过敏原含量;（2）确定天然存在的过敏原和/或低或低过敏原花生种质。使用来自有记录的花生过敏史的患者的人血清库，使用基本的ELISA方案来检测花生系中抗原的存在。两种天然存在的低变应原性或低变应原性种质被鉴定为PI 261942和PI 338386。两者均为瓦伦西亚市场类型，总过敏原含量显著低于（P ≤0.05）PI 119880（0.550）、PI 119876（0.415）和PI 118991（0.410）三种瓦伦西亚市场类型和PI 262111（0.485）弗吉尼亚市场类型。来自玻利维亚和巴拉圭的花生品系与来自委内瑞拉的花生品系过敏原含量差异显著（P ≤0.05）。四种市场类型的过敏原含量无显著差异。
Peanut is one of the most allergenic foods and reports of accidental ingestion of peanuts in unsuspected food are increasing. No information is available on the allergen content of peanut germplasm grown commercially and used in the food and confectionery industry. The objectives of this study were: (1) to determine the allergen contents of 34 peanut introductions (PI); and (2) to identify naturally occurring allergen-free and/or low or hypoallergenic peanuts germplasm. A basic ELISA protocol was utilized to detect the presence of antigens in the peanut lines using a pool of human sera from patients with documented history of peanut allergy. Two naturally occurring low, or hypo-allergenic germplasm were identified as PI 261942 and PI 338386. Both are Valencia market types with total allergen content significantly lower (P ≤0.05) than that of PI 119880 (0.550), PI 119876 (0.415) and PI 118991 (0.410) three Valencia market types and PI 262111 (0.485), a Virginia market type. No allergen-free PI was found. Allergen content of peanut lines from Bolivia and Paraguay were significantly (P ≤0.05) different to those from Venezuela. No significant difference was observed in the allergen content of the four market types.