Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato
Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato
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DOI：
10.1073/pnas.96.24.14153
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发表时间：
1999-11-23
影响因子：
11.1
通讯作者：
Staskawicz, BJ
中科院分区：
文献类型：
--
作者：
Tai, TH;Dahlbeck, D;Staskawicz, BJ
辣椒的Bs2抗性基因特异性识别并赋予对野油菜黄单胞菌致病变种（Xanthomonascampestris pv.）vesicatoria，含有相应的细菌无毒基因avrBs2。avrBs2参与病原体适合度及其在许多X.白菜型致病变种表明，Bs2基因在田间可能是持久的，并且当引入其它植物物种时提供抗性。采用定位克隆策略，分离Bs2位点，并通过在农杆菌介导的瞬时测定中与avrBs2共表达来鉴定该基因。一个单一的候选基因，预测编码的核苷酸结合位点富含亮氨酸的重复类抗性基因的特征图案，被确定。该基因特异性地控制过敏反应时，瞬时表达在敏感的辣椒和番茄线，并在非宿主物种，本塞姆氏烟草，并被指定为BS2。Bs2在稳定的转基因番茄中的功能性表达支持其在其他茄科植物物种中作为抗性来源的用途。
The Bs2 resistance gene of pepper specifically recognizes and confers resistance to strains of Xanthomonas campestris pv. vesicatoria that contain the corresponding bacterial avirulence gene, avrBs2. The involvement of avrBs2 in pathogen fitness and its prevalence in many X. campestris pathovars suggests that the Bs2 gene may be durable in the field and provide resistance when introduced into other plant species. Employing a positional cloning strategy, the Bs2 locus was isolated and the gene was identified by coexpression with avrBs2 in an Agrobacterium-mediated transient assay. A single candidate gene, predicted to encode motifs characteristic of the nucleotide binding site-leucine-rich repeat class of resistance genes, was identified. This gene specifically controlled the hypersensitive response when transiently expressed in susceptible pepper and tomato lines and in a nonhost species, Nicotiana benthamiana, and was designated as Bs2. Functional expression of Bs2 in stable transgenic tomatoes supports its use as a source of resistance in other Solanaceous plant species.