Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization
Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization
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DOI：
10.1016/s1097-2765(00)80295-6
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发表时间：
1998-12-01
期刊：
影响因子：
16
通讯作者：
Rouault, TA
中科院分区：
文献类型：
--
作者：
Land, T;Rouault, TA
铁硫簇是细胞中许多酶的功能所需的辅基，包括在呼吸、光合作用和固氮中重要的酶。在这里，我们报告克隆的人类同系物的NifS，半胱氨酸脱硫酶，建议提供无机硫的铁硫簇，在人类细胞中，不同形式的NifS，本地化的线粒体或细胞质和细胞核ave合成从一个单一的转录：通过启动et;替代的框内AUG，和起始位点的选择不同的介质或细胞质的pn。因此，一种新形式的翻译调节允许NifS蛋白响应于代谢状态的变化而快速重新分布到细胞的不同隔室中。
Iron-sulfur clusters are prosthetic groups that are required for the function of numerous enzymes in the cell, including enzymes important in respiration, photosynthesis, and nitrogen fixation. Here we report cloning of the human homolog of NifS, a cysteine desulfurase that is proposed to supply the inorganic sulfur in iron-sulfur clusters, In human cells, different forms of NifS that localize either to mitochondria or to the cytosol and nucleus ave synthesized from a single transcript: through initiation et;alternative in-frame AUGs, and initiation site selection varies according to the pn of the medium or cytosol. Thus, a novel form of translational regulation permits rapid redistribution of NifS proteins into different compartments of the cell in response to changes in metabolic status.