Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics
Multiple independent origins of Shigella clones of Escherichia coli and convergent evolution of many of their characteristics
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DOI：
10.1073/pnas.180094797
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发表时间：
2000-09-12
影响因子：
11.1
通讯作者：
Reeves, PR
中科院分区：
文献类型：
--
作者：
Pupo, GM;Lan, RT;Reeves, PR
对志贺氏菌属4个传统菌种(亚群)--痢疾杆菌、福氏志贺氏菌、博伊迪氏志贺菌和宋内氏志贺氏菌的4个血清型的46株志贺氏菌的进化关系进行了分析，结果表明，志贺氏菌属志贺氏菌属的4个传统菌种(亚群)的8个看家基因分别位于染色体的4个区域。分析显示，每个地区的进化模式非常相似。鉴定了三组菌株，每组包括来自不同亚群的菌株。群1包含大多数博伊迪和痢疾杆菌菌株(B1-4、B6、B8、B10、B14和B18；以及D3-7)。D9和D11-13)加上Flexneri 6和6A。簇2包含7个Boydii菌株(B5、B7、B9)。B11。B15。群3包括一株Boydii株(B12)和1-5型福氏杆菌。宋内氏杆菌和三株痢疾杆菌(d1、d8和d10)不在这三个主要聚集区之外，但显然在大肠杆菌内。Boydii 13与大肠杆菌有较远的亲缘关系。志贺氏菌与其他致病形式的大肠杆菌一样，并不具有单一的进化起源，这表明志贺氏菌表型特性的趋同进化。我们估计志贺氏菌的三个主要聚集性是在过去的3.5万到27万年内进化的，这表明志贺氏菌病是人类早期的传染病之一。
The evolutionary relationships of 46 Shigella strains representing each of the serotypes belonging to the four traditional Shigella species (subgroups), Dysenteriae, Flexneri, Boydii, and Sonnei, were determined by sequencing of eight housekeeping genes in four regions of the chromosome. Analysis revealed a very similar evolutionary pattern for each region. Three clusters of strains were identified, each including strains from different subgroups. Cluster 1 contains the majority of Boydii and Dysenteriae strains (B1-4, B6, B8, B10, B14, and B18; and D3-7. D9, and D11-13) plus Flexneri 6 and 6A. Cluster 2 contains seven Boydii strains (B5, B7, B9. B11. B15. B16, and B17) and Dysenteriae 2. Cluster 3 contains one Boydii strain (B12) and the Flexneri serotypes 1-5 strains. sonnei and three Dysenteriae strains (D1, D8, and D10) are outside of the three main clusters but, nonetheless, are clearly within Escherichia coli. Boydii 13 was found to be distantly related to E. coli. shigella strains, like the other pathogenic forms of E. coli, do not have a single evolutionary origin, indicating convergent evolution of Shigella phenotypic properties. We estimate the three main Shigella clusters to have evolved within the last 35,000 to 270,000 years, suggesting that shigellosis was one of the early infectious diseases of humans.