A cellular cofactor for the constitutive transport element of type D retrovirus.
A cellular cofactor for the constitutive transport element of type D retrovirus.
复制标题
DOI：
10.1126/science.276.5317.1412
复制
发表时间：
1997-05
期刊：
影响因子：
56.9
通讯作者：
H. Tang;G. Gaietta;W. Fischer;Mark Ellisman;F. Wong-Staal
中科院分区：
文献类型：
--
作者：
H. Tang;G. Gaietta;W. Fischer;Mark Ellisman;F. Wong-Staal
一种与猴逆转录病毒的组成型转运元件（CTE）特异性相互作用的人核蛋白被鉴定为腺苷5 '-三磷酸依赖性RNA解旋酶A。该蛋白可以结合功能CTE，但不结合失活CTE突变体。解旋酶A与CTE的相互作用不同于先前描述的这种蛋白质的解旋酶活性。解旋酶A在转录抑制剂存在下或在瞬时过表达含CTE RNA的细胞中从细胞核穿梭至细胞质。在结合原位杂交和免疫染色的实验中观察到解旋酶A和CTE的体内共定位。这些结果表明，解旋酶A在含CTE的RNA的核输出中起作用。
A human nuclear protein that specifically interacts with the constitutive transport element (CTE) of simian retrovirus was identified as adenosine 5'-triphosphate-dependent RNA helicase A. This protein could bind to functional CTE but not to inactive CTE mutants. The interaction of helicase A with CTE was distinct from previously described helicase activity of this protein. Helicase A shuttled from the nucleus to the cytoplasm in the presence of a transcription inhibitor or in cells transiently overexpressing CTE-containing RNA. In vivo colocalization of helicase A and CTE was observed in experiments that combined in situ hybridization and immunostaining. These results suggest that helicase A plays a role in the nuclear export of CTE-containing RNA.