EXTRALUMINAL RECORDING OF OVIDUCTAL CONTRACTIONS IN UNANESTHETIZED RABBIT
EXTRALUMINAL RECORDING OF OVIDUCTAL CONTRACTIONS IN UNANESTHETIZED RABBIT
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DOI:
10.1152/jappl.1971.31.5.768
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发表时间:
1971-01-01
影响因子:
3.3
通讯作者:
MAISTRELLO, I
中科院分区:
文献类型:
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作者:
MAISTRELLO, I
METHODSConstruction of transducer. The strain gauge portion of the transducer was made with medical-grade silicone rubber tubing (Silastic, Dow-Corning) having an internal and external diameter of 0.30 and 0.64 mm, respectively. The tubing was cut to a length of 16 mm and filled with mercury. A copper wire of 4 mm in diameter was enclosed in each end of the tube and acted as a plug for the mercury. One wire was 40 mm in length and the other 15 mm. The wires were then covered with silicone tubing previously soaked in xylene to enlarge the diameter. This tubing was pushed beyond the end of the tube filled with mercury(Fig.1B). As the xylene evaporated the tubing returned to its original size thus causing a tight seal of the mercury-copper contact and mercury leakage was prevented. The other ends of the copper wires were soldered to 0.127 mm2 polyvinyl chloride-insulated stranded copper lead (Datwyler Co.). The solder junction was coated with an insulating varnish and both the junctions and the polyvinyl chloride-insulated leads were covered with Silastic tubing (0.64 mm id, 1.19 mm od). This latter operation was facilitated by placing the Silastic tube in xylene. The mercury strain gauge was held in position within a U-shaped mounting apparatus(Fig. 1A). Horizontal holes were made in this apparatus for the passage of the copper wires. The-longer wire traversed the mounting apparatus and exited on the same side as the shorter one. The wires were fixed in position by adjusting the corresponding screws so that there was a slackening in the mercury tube resulting in a slight concavity between the lateral arms as shown by the measurements in Fig. 2A; therefore no initial tension was placed upon the mercury tube. Two vertical holes were drilled in each of the lateral arms for the passage of sutures for securing the mounting apparatus to the abdominal wall. The wires leading from the transducer were soldered to a miniature female Amphenol connector(type 126-22 1). The solder junctions were protected from fluid by an acrylic plastic. The mounting apparatus and the acrylic plastic were covered with a room temperature-during silicone rubber (Silastic 583 RTV). The average useful life of the transducer was 12 days. The cause of gauge failure was usually due to a break or separation of the mercury in the gauge itself.