The plasma membrane calcium pump: Recent developments and future perspectives
The plasma membrane calcium pump: Recent developments and future perspectives
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DOI：
10.1007/bf01952107
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发表时间：
1996-12-01
期刊：
影响因子：
--
通讯作者：
Guerini, D
中科院分区：
文献类型：
--
作者：
Carafoli, E;GarciaMartin, E;Guerini, D
质膜Ca2+泵（PMCA）是由许多药物调节。最重要的是钙调素（CaM），它与位于泵的c端部分的结构域结合，将其从活性位点旁边的自抑制位点移除。cam结合域之前有一个酸性序列，其中包含内质网保留的隐藏信号。PMCA和内质网（SERCA）泵的嵌合体揭示了SERCA泵的前45个残基中存在强烈的内质网保留信号。PMCA泵的四个基因产物是已知的：其中两个（1和4）是普遍表达的，两个（2和3）是神经细胞特异性的，可能是由它们的激活引起的。诱变工作已经确定了泵的三个跨膜域的四个残基，这可能是反式蛋白Ca2+路径的组成部分。其中两个残基的突变改变了泵的膜靶向性。
The Ca2+ pump of the plasma membrane (PMCA) is regulated by a number of agents. The most important is calmodulin (CaM), which binds to a domain located in the C-terminal portion of the pump, removing it from an autoinhibitory site next to the active site. The CaM-binding domain is preceded by an acidic sequence which contains a hidden signal for endoplasmic reticulum (ER) retention. Chimeras of the PMCA and endoplasmic reticulum (SERCA) pumps have revealed the presence of a strong signal for ER retention in the first 45 residues of the SERCA pump. Four gene products of the PMCA pump are known: two of them (1 and 4) are ubiquitously expressed, two (2 and 3) are specific for nerve cells and may be induced by their activation. Mutagenesis work has identified four residues in three of the transmembrane domains of the pump which may be components of the trans-protein Ca2+ path. The mutation of two of these residues alters the membrane targeting of the pump.