RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: A conserved mechanism for control of DNA recombination
RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: A conserved mechanism for control of DNA recombination
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DOI：
10.1016/s1097-2765(00)80354-8
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发表时间：
1999-05-01
期刊：
影响因子：
16
通讯作者：
Kowalczykowski, SC
中科院分区：
文献类型：
--
作者：
Harmon, FG;DiGate, RJ;Kowalczykowski, SC
E. coli RecQ蛋白是一种多功能解旋酶，其同源物包括S.酿酒酵母Sgs1解旋酶和H. sapiens pepsin和Blm解旋酶。在这里，我们表明RecQ解旋酶解旋共价闭合的双链DNA（dsDNA）底物，这种活动特异性地刺激E。coli拓扑异构酶III（Topo III）以完全链化dsDNA分子。我们认为这些蛋白质在功能上相互作用，它们的共同活性负责控制DNA重组。RecQ解旋酶对S.与其他RecQ和Topo III同源物一起以类似的能力起作用一致。这些发现突出了一种新的保守活性，该活性提供了对其他RecQ样解旋酶功能的深入了解。
E. coli RecQ protein is a multifunctional helicase with homologs that include the S. cerevisiae Sgs1 helicase and the H. sapiens Wrn and Blm helicases. Here we show that RecQ helicase unwinds a covalently closed double-stranded DNA (dsDNA) substrate and that this activity specifically stimulates E. coli topoisomerase III (Topo III) to fully catenate dsDNA molecules. We propose that these proteins functionally interact and that their shared activity is responsible for control of DNA recombination. RecQ helicase has a comparable effect on the Topo III homolog of S. cerevisiae, consistent with other RecQ and Topo III homologs acting together in a similar capacity. These findings highlight a novel, conserved activity that offers insight into the function of the other RecQ-like helicases.