Alterations in cochlear function during induced acute hyperinsulinemia in an animal model.
Alterations in cochlear function during induced acute hyperinsulinemia in an animal model.
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DOI：
10.1016/s1808-8694(15)30530-9
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发表时间：
2009-09
影响因子：
2.2
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中科院分区：
文献类型：
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继发于外周胰岛素抵抗的高胰岛素血症已被描述为耳蜗和前庭综合征最常见的病因。本实验采用经鼓膜耳蜗电图（EcochG）记录并分析了急性高胰岛素血症动物模型诱发听觉电位的变化。6只成年雄性绵羊随机分为2组。在全身麻醉下将动物送至EcochG，并采集外周血样品以测量胰岛素和胰岛素血症。干预组动物（n=3）接受常规人胰岛素IV（0.1 U/kg）。对照组（n=3）给予生理盐水。在90分钟内以10分钟间隔记录诱发电位，同时测量血糖和胰岛素血症。与对照组相比，干预组的动作电位振幅出现进行性抑制（p=0.001）。数据强烈表明，急性诱导的高胰岛素血症抑制耳蜗功能。结果可能归因于血管纹中Na+K+ ATP酶活性的丧失，导致耳蜗内电位的丧失和随后耳蜗毛细胞以及颅神经VIII听觉部分的神经细胞的去极化。
Hyperinsulinism secondary to peripheral insulin resistance has been described as the most frequent etiologic factor in cochlear and vestibular syndromes. This experimental study recorded and analyzed evoked auditory potential changes using transtympanic electrocochleography (EcochG) during induced acute hyperinsulinism in an animal model. Six adult male sheep were randomly divided into 2 groups. The animals were submitted to EcochG under general anesthesia, and a peripheral blood sample was collected to measure glycemia and insulinemia. Animals in the intervention group (n=3) received regular human insulin IV (0.1 U/kg). The control group (n=3) received saline solution. Glycemia and insulinemia were measured simultaneously with the recording of evoked potentials at 10-minute intervals during 90 minutes. The intervention group experienced a progressive suppression in action potential amplitude when compared to the control group (p=0.001). Data strongly suggest that acute induced hyperinsulinism suppresses cochlear function. Results may be attributed to loss of Na+K+ATPase activity in the stria vascularis, leading to loss of endocochlear potential and subsequent depolarization of cochlear hair cells as well as of neural cells in the auditory portion of cranial nerve VIII.