An Example of Genetically Distinct HIV Type 1 Variants in Cerebrospinal Fluid and Plasma During Suppressive Therapy
An Example of Genetically Distinct HIV Type 1 Variants in Cerebrospinal Fluid and Plasma During Suppressive Therapy
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DOI：
10.1093/infdis/jit805
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发表时间：
2014-05-15
影响因子：
6.4
通讯作者：
Palmer, Sarah
中科院分区：
文献类型：
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作者：
Dahl, Viktor;Gisslen, Magnus;Palmer, Sarah
我们对从70份脑脊液（CSF）标本和29份血浆标本中回收的人类免疫缺陷病毒1型（HIV-1）基因组进行了测序，并对17例接受抑制性治疗的受试者在治疗开始前获得的相应样本进行了测序。CSF序列比血浆序列更多的是超突变体。我们测定了治疗开始后从2例受试者中获得的标本的CSF序列和血浆序列。在一名受试者中，我们发现了遗传上不同的CSF和血浆序列，表明它们来自抑制治疗期间来自2个不同隔室的HIV-1，其中一个可能是中枢神经系统。此外，治疗期间CSF中几乎没有病毒进化的证据，表明病毒持续复制不是中枢神经系统中病毒持续存在的主要原因。
We sequenced the genome of human immunodeficiency virus type 1 (HIV-1) recovered from 70 cerebrospinal fluid (CSF) specimens and 29 plasma samples and corresponding samples obtained before treatment initiation from 17 subjects receiving suppressive therapy. More CSF sequences than plasma sequences were hypermutants. We determined CSF sequences and plasma sequences in specimens obtained from 2 subjects after treatment initiation. In one subject, we found genetically distinct CSF and plasma sequences, indicating that they came from HIV-1 from 2 different compartments, one potentially the central nervous system, during suppressive therapy. In addition, there was little evidence of viral evolution in the CSF during therapy, suggesting that continuous virus replication is not the major cause of viral persistence in the central nervous system.