PHOTORESPIRATION AND LIGHT ACT IN CONCERT TO REGULATE THE EXPRESSION OF THE NUCLEAR GENE FOR CHLOROPLAST GLUTAMINE-SYNTHETASE
PHOTORESPIRATION AND LIGHT ACT IN CONCERT TO REGULATE THE EXPRESSION OF THE NUCLEAR GENE FOR CHLOROPLAST GLUTAMINE-SYNTHETASE
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DOI：
10.1105/tpc.1.2.241
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发表时间：
1989-02-01
期刊：
影响因子：
11.6
通讯作者：
CORUZZI, GM
中科院分区：
文献类型：
--
作者：
EDWARDS, JW;CORUZZI, GM
在豌豆中，不同的叶绿体和胞质形式的谷氨酰胺合成酶（GS）由在体内差异表达的同源核基因编码（Tingey，S. V.，Tsai，F.-是的，爱德华兹，J.W.，步行者，E.L.，和Coruzzi，G.m. [1988年]。J.Biol.Chem.263，9651-9657）。在叶片中，光选择性地影响叶绿体GS 2的核基因的表达。用红光或白色光处理的黄化植物中GS 2 mRNA最大水平的差异表明，只有部分白光诱导的GS 2 mRNA积累是由于光敏色素介导的反应。GS 2 mRNA积累的动力学响应于白光照明的黄化或暗adpated绿色platns表明，GS 2 mRNA积累更迅速的植物含有成熟的，光合能力的叶绿体。GS 2 mRNA水平受叶绿体代谢状态影响的其他证据涉及在导致产生光呼吸氨的条件下生长的植物中GS 2 mRNA的选择性诱导。这些结果表明，光诱导GS 2 mRNA在叶片中的积累是光敏色素的作用以及光诱导叶绿体代谢变化的结果。
In Pisum sativum, distinct chloroplast and cytosolic forms of glutamine synthetase (GS) are encoded by homologous nuclear genes that are differentially expressed in vivo (Tingey, S.V., Tsai, F.-Y., Edwards, J.W., Walker, E.L., and Coruzzi, G.m. [1988]. J. Biol. Chem. 263, 9651-9657). In leaves, light selectively affects the expression of the nuclear gene for chloroplast GS2. Differences in the maximal levels of GS2 mRNA in etiolated plants treated with red or white light indicate that only part of the white-light-induced accumulation of GS2 mRNA is due to a phytochrome-mediated response. The kinetics of GS2 mRNA accumulation in response to white-light illumination of etiolated or dark-adpated green platns indicates that GS2 mRNA accumulates more rapidly in plants containing mature, photosynthetically competent chloroplasts. Other evidence that GS2 mRNA levels are affected by the metabolic status of chloroplasts concerns the selective induction of GS2 mRNA in plants grown under conditions that result in the production of photorespiratory ammonia. These results indicate that the light-induced accumulation of GS2 mRNA in leaves results from the action of phytochrome as well as light-induced changes in chloroplast metabolism.