The Unexpected Reduction of the Vinyl Group of Chlorophyll b by Sodium Borohydride in Methanolic Extracts of Maize Leaves and Its Inhibition by 8-Hydroxyquinoline
The Unexpected Reduction of the Vinyl Group of Chlorophyll b by Sodium Borohydride in Methanolic Extracts of Maize Leaves and Its Inhibition by 8-Hydroxyquinoline
复制标题
硼氢化钠对玉米叶甲醇提取物中叶绿素 b 乙烯基的意外还原及其 8-羟基喹啉的抑制作用
DOI：
10.1515/znc-1993-9-1011
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
H. Scheer
中科院分区：
文献类型：
--
作者：
R. J. Porra;W. Schäfer;E. Cmiel;I. Katheder;H. Scheer
在含NaBH4的甲醇还原条件下快速提取玉米叶片叶绿素时，叶绿素a保持不变，而叶绿素b生成[7-羟甲基]-叶绿素b，叶绿素b的3-乙烯基也被大量还原，最高可达60%。[3-乙基]-[7-羟甲基]-叶绿素b。这是出乎意料的，因为当分离的叶绿素b与甲醇硼氢化物以相同的方式处理时，3-乙烯基的还原不会发生。在相同的提取条件下，叶绿素a的乙烯基没有减少，这表明在C-7上存在甲酰基或羟乙基是必要的。8-羟基喹啉和NaBH4等摩尔浓度（16.5 m m）的存在强烈抑制叶片提取物中叶绿素b 3-乙烯基的还原
Abstract During rapid extraction of chlorophylls from maize leaves under reducing conditions with methanol containing NaBH4, chlorophyll a remained unchanged but chlorophyll b yielded [7-hydroxymethyl]-chlorophyll b. The 3-vinyl group of chlorophyll b was also reduced forming significant am ounts, up to 60%, of [3-ethyl]-[7-hydroxymethyl]-chlorophyll b. This was unexpected since this reduction of the 3-vinyl group does not occur when isolated chlorophyll b is treated in an identical manner with methanolic borohydride. The vinyl-group of chlorophyll a is not reduced during the same extraction conditions suggesting that the presence of a formyl or hydroxyethyl group at C-7 is necessary. The presence of 8-hydroxyquinoline and NaBH4 in equimolar (16.5 m M) concentrations strongly inhibits the reduction of the 3-vinyl group of chlorophyll b in leaf extracts