HISTOCHEMISTRY OF CONNECTIVE-TISSUE CELLS IN SUBCUTANEOUS ADIPOSE-TISSUE OF NORMAL AND DECAPITATED PIG FETUSES
HISTOCHEMISTRY OF CONNECTIVE-TISSUE CELLS IN SUBCUTANEOUS ADIPOSE-TISSUE OF NORMAL AND DECAPITATED PIG FETUSES
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DOI：
10.1159/000145835
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
HAUSMAN, GJ
中科院分区：
文献类型：
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作者：
HAUSMAN, GJ
在猪和大鼠中，在组织化学和形态学上与成纤维细胞不同的结缔组织细胞位于发育中的毛囊周围。未成熟脂肪组织仅限于大鼠皮下组织中完全下降的毛囊周围的小区域。在这里，检查胎猪皮下组织中大神经和血管周围的结缔组织中脂肪细胞典型酶的存在。研究了断头猪胎的样品，以便检查激素谱改变的影响。从妊娠65、70、85、90、110和112天的胎仔中采集背部皮下脂肪组织样本。在妊娠45天时，在子宫内处死胎仔，并在妊娠110天时从这些胎仔中获得脂肪组织样本。在较大（> 70天）的胎儿中，观察到大血管和神经的生长与脂肪细胞簇发育之间存在密切的空间关系。与脂肪细胞簇相邻的结缔组织细胞在组织化学上与脂肪细胞相同。在年轻胎儿中，反应性结缔组织细胞（组织化学上与脂肪细胞相同）的脂质组织化学是可变的。在所有110和112日龄的胎儿，反应细胞含有脂质，反应细胞在断头胎儿没有脂质。反应性结缔组织细胞不与毛细血管相连，不含基底膜。这些细胞的组织化学表明，它们像脂肪细胞一样对特定的激素或代谢谱作出反应。与脂肪细胞簇相邻的结缔组织细胞可能代表未分化状态和成熟脂肪细胞之间的脂肪细胞分化的不同阶段。
Connective tissue cells that are histochemically and morphologically distinct from fibroblasts are localized around developing hair follicles in the pig and rat. Immature adipose tissue is limited to small areas immediately around fully descended hair follicles in the rat hypodermis. Here, connective tissue around large nerves and blood vessels in fetal pig subcutaneous tissue was examined for the presence of enzymes typical of adipocytes. Samples from decapitated pig fetuses were studied so that the effects of an altered hormonal profile could be examined. Samples of dorsal subcutaneous adipose tissue were obtained from fetuses at 65, 70, 85, 90, 110 and 112 days of gestation. Fetuses were decapitated in utero at 45 days of gestation, and adipose tissue samples were obtained from these fetuses at 110 days of gestation. A close spatial relationship was observed between the growth of large blood vessels and nerves and fat cell cluster development in the older (> 70 days) fetuses. Connective tissue cells that were contiguous with fat cell clusters were histochemically identical to adipocytes. The lipid histochemistry of the reactive connective tissue cells (histochemically identical to adipocyte) was variable in young fetuses. In all 110- and 112-day-old fetuses, the reactive cells contained lipid; the reactive cells in decapitated fetuses were devoid of lipid. The reactive connective tissue cells were not associated with capillaries and did not contain basement membranes. Histochemistry of these cells suggests that they respond to a particular hormonal or metabolic profile as do adipocytes. Connective tissue cells contiguous with fat cell clusters may represent a distinct stage of adipocyte differentiation between the undifferentiated state and the mature adipocyte.