The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1
The CaCTR1 gene is required for high-affinity iron uptake and is transcriptionally controlled by a copper-sensing transactivator encoded by CaMAC1
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DOI：
10.1099/mic.0.27004-0
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发表时间：
2004-07-01
期刊：
影响因子：
2.8
通讯作者：
Cashmore, AM
中科院分区：
文献类型：
--
作者：
Marvin, ME;Mason, RP;Cashmore, AM
已知白色念珠菌从宿主的不利环境中获得铁的能力是毒力所必需的，并且似乎使用与酿酒酵母所描述的系统类似的系统来实现。In S.在酿酒酵母中，高亲和力的铁吸收依赖于铜的获得。作者以前已经确定了一个C。白色念珠菌基因（CaCTR 1）编码的铜转运蛋白。该基因的缺失导致突变株主要以假菌丝生长，并在低铜条件下显示异常形态。本文证明了C.白色念珠菌是由低铜条件诱导的，这在Cactr 1-null菌株中增强。它还表明，CaCTR 1的缺失导致铁吸收缺陷。In S.在酿酒酵母中，促进高亲和力铜吸收的基因由铜敏感反式激活因子ScMac 1 p控制。作者现在已经确定了一个C。白色念珠菌基因（CaMAC 1），编码铜敏感反式激活因子。一个Camac 1无效突变体显示表型相似的Cactr 1无效突变体，并没有检测到的CaCTR 1转录在低铜条件下。提出了C.白念珠菌对还原性铁的摄取是必需的，并且以与S.啤酒。
The ability of Candida albicans to acquire iron from the hostile environment of the host is known to be necessary for virulence and appears to be achieved using a similar system to that described for Saccharomyces cerevisiae. In S. cerevisiae, high-affinity iron uptake is dependent upon the acquisition of copper. The authors have previously identified a C. albicans gene (CaCTR1) that encodes a copper transporter. Deletion of this gene results in a mutant strain that grows predominantly as pseudohyphae and displays aberrant morphology in low-copper conditions. This paper demonstrates that invasive growth by C. albicans is induced by low-copper conditions and that this is augmented in a Cactr1-null strain. It also shows that deletion of CaCTR1 results in defective iron uptake. In S. cerevisiae, genes that facilitate high-affinity copper uptake are controlled by a copper-sensing transactivator, ScMac1p. The authors have now identified a C. albicans gene (CaMAC1) that encodes a copper-sensing transactivator. A Camac1-null mutant displays phenotypes similar to those of a Cactr1-null mutant and has no detectable CaCTR1 transcripts in low-copper conditions. It is proposed that high-affinity copper uptake by C. albicans is necessary for reductive iron uptake and is transcriptionally controlled by CaMac1 p in a similar manner to that in S. cerevisiae.