Infrared reflection-absorption spectroscopy and polarization-modulated infrared reflection-absorption spectroscopy studies of the aequorin langmuir monolayer.
Infrared reflection-absorption spectroscopy and polarization-modulated infrared reflection-absorption spectroscopy studies of the aequorin langmuir monolayer.
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水母发光蛋白朗缪尔单层的红外反射吸收光谱和偏振调制红外反射吸收光谱研究。
DOI：
10.1021/jp710953j
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
R. Leblanc
中科院分区：
文献类型：
--
作者：
Chengshan Wang;M. Mićić;M. Ensor;S. Daunert;R. Leblanc
通过红外反射吸收光谱 (IRRAS) 和偏振调制 IRRAS 技术研究了水母发光蛋白和脱辅基水母发光蛋白的 Langmuir 单层。水母发光蛋白 Langmuir 单层中的 α 螺旋在零表面压力下平行于空气-水界面。当表面压力增加到15 mN.(m-1)时，α螺旋变得倾斜并且转弯变得平行于空气-水界面。对于脱辅基水母发光蛋白，α螺旋在0 mN.m(-1)处也平行于空气-水界面。然而，α-螺旋变得倾斜，并且转弯在 5 mN.m(-1) 时很快变得与空气-水界面平行。随着脱辅基水母蛋白朗缪尔单层的进一步压缩，方向保持不变。水母发光蛋白和脱辅基水母发光蛋白在空气-水界面上的不同行为可以通过以下事实来解释：水母发光蛋白在空气-水界面上形成二聚体，而脱辅基水母发光蛋白是单体。由于 Langmuir 单层的压缩，二聚体更难以倾斜。
The Langmuir monolayer of aequorin and apoaequorin was studied by infrared reflection-absorption spectroscopy (IRRAS) and polarization-modulated IRRAS techniques. The alpha-helices in the aequorin Langmuir monolayer were parallel to the air-water interface at zero surface pressure. When the surface pressure increased to 15 mN.(m-1), the alpha-helices became tilted and the turns became parallel to the air-water interface. As for apoaequorin, the alpha-helices were also parallel to the air-water interface at 0 mN.m(-1). However, the alpha-helix became tilted and the turns became parallel to the air-water interface quickly at 5 mN.m(-1). With further compression of the apoaequorin Langmuir monolayer, the orientation remained the same. The different behaviors of aequorin and apoaequorin at the air-water interface were explained by the fact that aequorin formed dimers at the air-water interface but apoaequorin was a monomer. It is more difficult for a dimer to be tilted by the compression of the Langmuir monolayer.