Changes in metabolite profiles in Norway spruce shoot tips during short-day induced winter bud development and long-day induced bud flush
Changes in metabolite profiles in Norway spruce shoot tips during short-day induced winter bud development and long-day induced bud flush
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DOI：
10.1007/s11306-014-0646-x
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发表时间：
2014-10-01
期刊：
影响因子：
3.6
通讯作者：
Olsen, Jorunn Elisabeth
中科院分区：
文献类型：
--
作者：
Lee, Yeon Kyeong;Alexander, Danny;Olsen, Jorunn Elisabeth
光周期的季节变化是影响树木生长的主要环境信号。长日照（LD）维持生长，而短日照（SD）则诱导冬芽形成。在这方面，仅在针叶树中对代谢组反应进行了有限程度的研究。在这里，我们确定了针叶树挪威云杉在过渡到 SD 后以及重新转移到 LD 后诱导花蕾冲洗后代谢物谱的变化。 SD 1 周后，代谢物谱的初始变化是可见的，但对于大多数化合物来说，变化幅度很小。然而，抗坏血酸含量大幅降低，并且几种与能量代谢相关的化合物、次级代谢产物、核苷、氨基酸和脂质经常暂时增加。 8 周后，观察到 SD 发生显着变化；正常的冬芽含有大量与胁迫耐受和硬化相关的ABA、抗氧化剂、黄酮类、萜类、苯丙素、糖、氨基酸和脂类，以及与能量代谢相关的核苷和代谢物含量较低。重新转移到 LD 一周后，代谢组通常与长期 SD 下相对相似，除了例如尿酸盐增加，ABA 和氧化型谷胱甘肽明显减少。两周后，出现花蕾，代谢物谱与转移至 SD 之前的情况相似。因此，这项研究揭示了挪威云杉代谢组对日长变化的全面调节，表明在 SD 诱导的芽形成下，抗逆性显着增加，而在 LD 中芽冲洗时则逆转。
The seasonal change in photoperiod is a primary environmental signal influencing tree growth. Long days (LD) sustain growth, whereas short days (SD) induce winter bud formation. In this respect, metabolomic responses have been studied to a limited extent only in conifers. Here we identified changes in metabolite profile in the conifer Norway spruce after transition to SD and following re-transfer to LDs inducing bud flush. After 1 week in SD initial changes in metabolite profile was visible but for the majority of compounds magnitudes of changes were small. However, the ascorbate content was strongly reduced and there were often temporary increases in several energy metabolism-related compounds, secondary metabolites, nucleosides, amino acids and lipids. After 8 weeks in SD substantial changes were observed; proper winter buds had high pools of ABA, antioxidants, flavonoids, terpenoids, phenylpropanoids, sugars, amino acids and lipids related to stress tolerance and hardening, and low levels of nucleosides and metabolites in energy metabolism. One week after re-transfer to LD the metabolome was generally relatively similar to under long-term SD, except e.g. increased urate and strongly decreased ABA and oxidized glutathione. Two weeks later, bud flush had occurred, and the metabolite profile resembled the situation before transfer to SD. This study thus revealed comprehensive modulation of the metabolome in Norway spruce in response to a day length shift, indicating substantially increased stress resistance under SD-induced bud set, and reversal upon bud flush in LD.