CLONING AND CHROMOSOMAL MAPPING OF 3 NOVEL GENES, GPR9, GPR10, AND GPR14 ENCODING RECEPTORS RELATED TO INTERLEUKIN-8, NEUROPEPTIDE-Y, AND SOMATOSTATIN RECEPTORS
CLONING AND CHROMOSOMAL MAPPING OF 3 NOVEL GENES, GPR9, GPR10, AND GPR14 ENCODING RECEPTORS RELATED TO INTERLEUKIN-8, NEUROPEPTIDE-Y, AND SOMATOSTATIN RECEPTORS
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DOI：
10.1006/geno.1995.9996
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发表时间：
1995-09-20
期刊：
影响因子：
4.4
通讯作者：
DOCHERTY, JM
中科院分区：
文献类型：
--
作者：
MARCHESE, A;HEIBER, M;DOCHERTY, JM
我们采用聚合酶链反应和基因组DNA文库筛选克隆新的人类基因，GPR 9和GPR 10，和大鼠基因，GPR 14。GPR 9、GPR 10和GPR 14各自编码G蛋白偶联受体。GPR 10和GPR 14在其编码区内是无内含子的，而GPR 9含有至少一个内含子。由GPR 9编码的受体与人IL-8受体类型B具有最高的同一性（总体为38%，跨膜区为53%），其次是IL-8受体类型A（总体为36%，跨膜区为51%）。GPR 10编码与神经肽Y受体具有最高同一性的受体（总体为31%，跨膜结构域为46%）。由GPR 14编码的受体与生长抑素受体SSTR 4具有最高的同一性（总体27%，跨膜结构域41%）。荧光原位杂交分析将GPR 9定位于染色体8p11.2-p12，GPR 10定位于染色体10q25.3-q26。(C)出版社：Academic Press
We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The receptor encoded by GPR9 shares the highest identity with human IL-8 receptor type B (38% overall and 53% in the transmembrane regions), followed by IL-8 receptor type A (36% overall and 51% in the transmembrane domains). GPR10 encodes a receptor that shares highest identity with the neuropeptide Y receptor (31% overall and 46% in the transmembrane domains). The receptor encoded by GPR14 shares highest identity with the somatostatin receptor SSTR 4 (27% overall and 41% in the transmembrane domains). Fluorescence in situ hybridization analysis localized GPR9 to chromosome 8p11.2-p12 and GPR10 to chromosome 10q25.3-q26. (C) 1995 Academic Press, Inc.